Interaction between D-glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase labeled by fluorescein-5'-isothiocyanate: evidence that the dye participates in the interaction

Biochemical and Biophysical Research Communications
M V SukhodoletsN K Nagradova

Abstract

An interaction of rabbit muscle D-glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase labeled with FITC was studied by following the changes in fluorescence intensity of the bound dye. The association between the two enzymes was found to be a rather slow process characterized by a second order rate constant of 1.1 +/- 0.2.10(3) M-1 s-1, the KD of the complex between apoenzymes being 3.2.10(-7) M. The stability of the complex increased upon increase of temperature and ionic strength of the medium, suggesting a hydrophobic character of association. The ligands which bind at the active centers of the two enzymes (NAD+, ATP, 3-phosphoglycerate) weakened the bienzyme association. Unlabeled 3-phosphoglycerate kinase was unable to displace the FITC-labeled enzyme from the complex. Taken together, the results indicate that interaction between D-glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase labeled by FITC is assisted by the dye, which may bind at nucleotide-binding sites of GPDH. No interaction was observed between the FITC-labeled 3-phosphoglycerate kinase and lactate dehydrogenase, which suggests that protein-protein interaction at specific "recognition" sites may be a prerequisite for the ...Continue Reading

References

Aug 1, 1979·The Biochemical Journal·N G OikonomakosA E Evangelopoulos
Apr 12, 1977·Biochimica Et Biophysica Acta·L L Somerville, F A Quiocho
Mar 2, 1988·Biochimica Et Biophysica Acta·R L Davis, J D Robinson
May 1, 1987·Archives of Biochemistry and Biophysics·C R Fleschner, N Kraus-Friedmann
Sep 30, 1987·Biochemical and Biophysical Research Communications·F OroszJ Ovádi
Nov 3, 1986·European Journal of Biochemistry·F Orosz, J Ovádi
Dec 1, 1970·European Journal of Biochemistry·W K Krietsch, T Bücher
Dec 14, 1984·Biochemical and Biophysical Research Communications·T L KirleyL K Lane
Sep 11, 1984·Biochimica Et Biophysica Acta·M V IvanovN K Nagradova
Jul 1, 1982·European Journal of Biochemistry·M V IvanovN K Nagradova

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Citations

Jan 11, 1996·Journal of Biochemical and Biophysical Methods·O P MalhotraA M Kayastha
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V I MuronetzT Korpela
Apr 29, 2008·Journal of Molecular Recognition : JMR·Andrew G GehringHerbert G Lebherz
Jan 15, 1995·European Journal of Biochemistry·O P MalhotraA M Kayastha

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