Interaction between human amphipathic apolipoproteins and amyloid beta-peptide: surface plasmon resonance studies

FEBS Letters
V V Shuvaev, G Siest

Abstract

Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) was quantified by plasmon resonance. The interactions were saturable and reversible. The results demonstrated a high affinity of the binding of amphipathic apolipoproteins to amyloid beta-peptide. On the other hand, only a small population of synthetic amyloid beta-peptide participated in the interaction. The apparent equilibrium dissociation constants K(D) were 10 nM for apoE3, 25 nM for apoA-I and 80 nM for apoA-II under physiological conditions. The affinity of the apoE3-amyloid beta-peptide binding was not affected by pH in the range 6.0-8.0 but was significantly increased by high salt concentration. ApoA-I mainly followed similar patterns. A major participation of hydrophobic forces in the binding of apoE3 and apoA-I to amyloid beta-peptide was suggested.

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Citations

Feb 25, 2000·Neuroscience Letters·V V Shuvaev, G Siest
Apr 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Philip B VergheseDavid M Holtzman
Jan 5, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·A R KoudinovY D Ivanov
Jan 7, 2014·Molecular Neurodegeneration·Leon M TaiMary Jo LaDu
Nov 21, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mitsutoshi SuganoHiroyoshi Ota
Nov 29, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·André F MartinsCarlos F G C Geraldes
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Dec 13, 2006·Neurobiology of Aging·Rodney T PerryWilliam D Hill
Dec 16, 1996·Annals of the New York Academy of Sciences·Y J KwonN R Relkin
Feb 26, 2015·Chemistry : a European Journal·André F MartinsCarlos F G C Geraldes
Mar 12, 2004·Biochemical and Biophysical Research Communications·Claudia TorricelliAdriana Pacini
Nov 23, 2005·Biochemistry·Hiroyoshi KakuyamaLars O Tjernberg

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