Interaction between Lactobacillus kefir and Saccharomyces lipolytica isolated from kefir grains: evidence for lectin-like activity of bacterial surface proteins

The Journal of Dairy Research
Marina Alejandra GolowczycGraciela Liliana De Antoni

Abstract

Several microbial interactions involving yeast and lactobacilli have been suggested in fermented products. Co-aggregation between Lactobacillus kefir and yeast Saccharomyces lipolytica isolated from kefir grains was studied by scanning electron microscopy and aggregation assays. Six out of twenty Lb. kefir strains were able to co-aggregate with Sacch. lipolytica CIDCA 812 and showed thermolabile non-covalently bound surface molecules involved in this interaction. Co-aggregation inhibition after Lb. kefir pre-treatment with 5 m-LiCl or 20 g SDS/l showed that bacterial S-layer proteins play an important role in this interaction. Presence of different sugar (mannose, sucrose and fructose) or yeast pre-treatment with sodium periodate inhibited co-aggregation between Lb. kefir and Sacch. lipolytica. Co-aggregating Lb. kefir strains were also able to agglutinate with human red blood cells and they lost this ability after treatment with 5 m-LiCl. These results and the capacity of purified S-layer proteins of Lb. kefir to haemagglutinate, strongly suggest that a lectin-like activity of bacterial surface proteins (S-layer) mediates the aggregation with yeast cells.

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Citations

May 17, 2013·Applied Microbiology and Biotechnology·Ulla Hynönen, Airi Palva
Dec 12, 2012·The Journal of Dairy Research·Patricia Araceli BollaGraciela Liliana De Antoni
Oct 31, 2013·The Journal of Dairy Research·Paula CarasiMaría de los Angeles Serradell
Mar 24, 2009·Antonie van Leeuwenhoek·Pablo MobiliGraciela L De Antoni
Jun 25, 2013·Journal of Bioscience and Bioengineering·Soichi FurukawaYasushi Morinaga
Sep 19, 2012·Food Microbiology·Sheng-Yao WangMing-Ju Chen
Dec 18, 2014·Bioscience, Biotechnology, and Biochemistry·Soichi FurukawaYasushi Morinaga
Mar 3, 2012·Biochemical and Biophysical Research Communications·Satoru HirayamaYasushi Morinaga
Feb 6, 2015·World Journal of Microbiology & Biotechnology·Esteban GerbinoAndrea Gómez-Zavaglia
Aug 9, 2016·Progress in Biophysics and Molecular Biology·Chaohua ZhuMing Sun
Sep 16, 2018·Journal of Applied Microbiology·A A BengoaA G Abraham
Sep 28, 2015·World Journal of Microbiology & Biotechnology·E GerbinoA Gómez-Zavaglia
Feb 6, 2020·Microorganisms·Fatemeh NejatiPeter Neubauer
Oct 17, 2018·Environmental Science and Pollution Research International·Yi XuLingzhan Miao
Dec 23, 2016·Antonie van Leeuwenhoek·Mariano MalamudMaría de Los Angeles Serradell
Jul 26, 2018·Antonie van Leeuwenhoek·Xue HanSai-Nan Zhao
Feb 7, 2019·Environmental Science and Pollution Research International·Yi XuLingzhan Miao

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