Interaction between lck and syk family tyrosine kinases in Fc gamma receptor-initiated activation of natural killer cells.

The Journal of Biological Chemistry
A T TingP J Leibson

Abstract

Ligation of the Fc gamma R on natural killer (NK) cells results in the tyrosine phosphorylation of multiple substrates critical for intracellular signaling and activation of NK cell effector functions. However, it remains unclear which nonreceptor protein-tyrosine kinases (PTK) participate in this process. In this report we demonstrate that Fc gamma R ligation induced the tyrosine phosphorylation and increased the catalytic activities of both syk family PTKs, ZAP-70, and syk. The phosphorylation of ZAP-70 and syk was enhanced markedly by overexpression of wild-type lck but not by a kinase-inactive mutant, suggesting that early Fc gamma R-initiated activation of lck results in the subsequent regulation of syk family PTKs. The regulatory interplay between src and syk family PTKs was emphasized further by the observation that lck overexpression enhanced the association of ZAP-70 with the zeta chain of the Fc gamma R complex. Additional analyses indicated that lck induced the subsequent tyrosine phosphorylation of phospholipase C (PLC)-gamma 2. Interestingly, the regulatory effects of lck on ZAP-70, syk, and PLC-gamma 2 could not be replaced by overexpression of either fyn or src, demonstrating a selective role for lck in effective...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J J O'SheaM J Smyth
May 1, 1992·The Journal of Experimental Medicine·U WirthmuellerJ V Ravetch
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J J O'SheaJ R Ortaldo
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A C ChanA Weiss
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Letourneur, R D Klausner
Jan 1, 1991·Annual Review of Immunology·J V Ravetch, J P Kinet
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K J EinspahrP J Leibson
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E K BarberC E Rudd
Jan 1, 1989·Advances in Immunology·G Trinchieri
May 1, 1994·The Journal of Experimental Medicine·T KurosakiH Yamamura
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·C CoutureT Mustelin
Jul 16, 1993·Cell·W KolanusB Seed
May 1, 1993·The Journal of Experimental Medicine·T W SalcedoB Perussia

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Citations

Oct 22, 2009·Neuromolecular Medicine·Eitan OkunThiruma V Arumugam
Oct 2, 2003·Journal of Molecular and Cellular Cardiology·Mark ShilkrutOfer Binah
Jun 1, 1997·Current Opinion in Immunology·J C Unkeless, J Jin
Aug 4, 1998·The Journal of Experimental Medicine·D D BilladeauP J Leibson
Sep 21, 2002·Molecular and Cellular Biology·Christine C HudsonRobert T Abraham
Apr 2, 2011·Journal of Innate Immunity·Yenan T BrycesonStephanie M Wood
Oct 9, 2002·The Journal of Clinical Investigation·Robin PariharWilliam E Carson
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·R PaoliniA Santoni
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·A H IdrisW M Yokoyama
Feb 12, 1998·The Journal of Experimental Medicine·K M BrumbaughP J Leibson
Nov 12, 2013·Critical Reviews in Oncology/hematology·Semra Paydas
Jan 20, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P F HalloranK S Famulski
Mar 28, 1997·The Journal of Biological Chemistry·C J Fitzer-AttasZ Eshhar
May 13, 2003·Nature Immunology·Daniel D BilladeauPaul J Leibson
Dec 22, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason L PughPeter Parham
Jun 28, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M D ParkesL G Hidalgo
Aug 16, 2002·Molecular Pharmacology·Fatih OzdenerJames L Daniel
Sep 5, 2008·Current Protocols in Cytometry·C C Chini, P J Leibson
Jul 5, 1996·The Journal of Biological Chemistry·B HaimovichR Greco
Jan 9, 1999·The Journal of Biological Chemistry·S MotoyaA Takeda
Mar 7, 2002·International Reviews of Immunology·K S Campbell, M Colonna
Mar 7, 2002·International Reviews of Immunology·A SulicaR B Herberman
Oct 9, 1998·The Journal of Biological Chemistry·B A BinstadtP J Leibson
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D D BilladeauP J Leibson
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie M RodaWilliam E Carson
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simona PisegnaAngela Santoni
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ilaria TassiMarco Colonna
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dianne KhuranaPaul J Leibson

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