Interaction Between Macrophage Migration Inhibitory Factor and CD74 in Human Immunodeficiency Virus Type I Infected Primary Monocyte-Derived Macrophages Triggers the Production of Proinflammatory Mediators and Enhances Infection of Unactivated CD4+ T Cells

Frontiers in Immunology
César TrifoneGabriela Turk

Abstract

Understanding the mechanisms of human immunodeficiency virus type I (HIV-1) pathogenesis would facilitate the identification of new therapeutic targets to control the infection in face of current antiretroviral therapy limitations. CD74 membrane expression is upregulated in HIV-1-infected cells and the magnitude of its modulation correlates with immune hyperactivation in HIV-infected individuals. In addition, plasma level of the CD74 activating ligand macrophage migration inhibitory factor (MIF) is increased in infected subjects. However, the role played by MIF/CD74 interaction in HIV pathogenesis remains unexplored. Here, we studied the effect of MIF/CD74 interaction on primary HIV-infected monocyte-derived macrophages (MDMs) and its implications for HIV immunopathogenesis. Confocal immunofluorescence analysis of CD74 and CD44 (the MIF signal transduction co-receptor) expression indicated that both molecules colocalized at the plasma membrane specifically in wild-type HIV-infected MDMs. Treatment of infected MDMs with MIF resulted in an MIF-dependent increase in TLR4 expression. Similarly, there was a dose-dependent increase in the production of IL-6, IL-8, TNFα, IL-1β, and sICAM compared to the no-MIF condition, specifically ...Continue Reading

References

Nov 20, 1996·AIDS Research and Human Retroviruses·V GiordanengoJ C Lefebvre
Jul 7, 1998·The Journal of Experimental Medicine·T W ChunA S Fauci
Jun 8, 1999·The Journal of Experimental Medicine·D UnutmazD R Littman
Sep 6, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E YamaguchiY Kawakami
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·P Stumptner-CuveletteP Benaroch
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert A MitchellRichard Bucala
Feb 21, 2002·Biochimica Et Biophysica Acta·Pamela Stumptner-Cuvelette, Philippe Benaroch
Mar 14, 2003·The Journal of Pathology·Kar Neng LaiHui Yao Lan
Jun 5, 2003·The Journal of Experimental Medicine·Lin LengRichard Bucala
Sep 23, 2003·Nature Reviews. Immunology·Thierry Calandra, Thierry Roger
Nov 23, 2006·Nature Medicine·Jason M BrenchleyDaniel C Douek
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brandon F KeeleGeorge M Shaw
Nov 15, 2008·Molecular Medicine·Xing-Xiang HeYousef Al-Abed
Dec 3, 2008·PloS One·Joseph N BrownMaureen M Goodenow
Apr 11, 2009·Retrovirology·Jan SchmökelFrank Kirchhoff
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edana CassolGuido Poli
Jun 3, 2009·The Journal of Experimental Medicine·Jesus F Salazar-GonzalezGeorge M Shaw
Jul 14, 2009·The Journal of Infectious Diseases·Gordon A AwandareDouglas J Perkins
Jul 16, 2009·BMC Cancer·Eva VerjansJürgen Bernhagen
Aug 8, 2009·Journal of Theoretical Biology·Ha Youn LeeAlan S Perelson
Apr 13, 2010·Retrovirology·Georges Herbein, Audrey Varin
Jun 4, 2010·Drug News & Perspectives·Gerrit GriebRichard Bucala
Aug 24, 2010·Bioorganic & Medicinal Chemistry Letters·Alissa A HareWilliam L Jorgensen
Sep 2, 2010·QJM : Monthly Journal of the Association of Physicians·H ConroyS C Donnelly

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
transfection
ELISA
flow

Software Mentioned

FV10
BD FACSDiva
FlowJO
Image J
GraphPad Prism
BD
FACSDiva

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