Interaction between Oc-1 and Lmx1a promotes ventral midbrain dopamine neural stem cells differentiation into dopamine neurons

Brain Research
Jian YuanDong-Bo Chen

Abstract

Recent studies have shown that Onecut (Oc) transcription factors may be involved in the early development of midbrain dopaminergic neurons (mdDA). The expression profile of Oc factors matches that of Lmx1a, an important intrinsic transcription factor in the development of mDA neuron. Moreover, the Wnt1-Lmx1a pathway controls the mdDA differentiation. However, their expression dynamics and molecular mechanisms remain to be determined. To address these issues, we hypothesize that cross-talk between Oc-1 and Lmx1a regulates the mdDA specification and differentiation through the canonical Wnt-β-catenin pathway. We found that Oc-1 and Lmx1a displayed a very similar expression profile from embryonic to adult ventral midbrain (VM) tissues. Oc-1 regulated the proliferation and differentiation of ventral midbrain neural stem cells (vmNSCs). Downregulation of Oc-1 decreased both transcript and protein level of Lmx1a. Oc-1 interacted with lmx1a in vmNSCs in vitro and in VM tissues in vivo. Knockdown of Lmx1a reduced the expression of Oc-1 and Wnt1 in vmNSCs. Inhibiting Wnt1 signaling in vmNSCs provoked similar responses. Our data suggested that Oc-1 interacts with Lmx1a to promote vmNSCs differentiation into dopamine neuron through Wnt1-L...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 28, 2006·Cell·Elisabet AnderssonJohan Ericson
Apr 25, 2006·Developmental Biology·Mahmud Bani-YaghoubMarianna Sikorska
Jul 11, 2006·The Journal of Physiology·Nilima Prakash, Wolfgang Wurst
Oct 13, 2006·Trends in Neurosciences·J Peter H Burbach, Marten P Smidt
Mar 3, 2007·Developmental Biology·Asa Abeliovich, Rachel Hammond
Jan 6, 2009·Nature Neuroscience·Milan JoksimovicRajeshwar B Awatramani
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stina FrilingJohan Ericson
Jun 23, 2011·Annual Review of Neuroscience·Nuria Flames, Oliver Hobert
Aug 11, 2011·The Journal of Comparative Neurology·Fuguo WuXiuqian Mu
Sep 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carol H YanSiew-Lan Ang
Nov 22, 2011·The Journal of Comparative Neurology·Agnès Espana, Frédéric Clotman
Apr 3, 2012·Annual Review of Neuroscience·Clemens Kiecker, Andrew Lumsden
Apr 27, 2012·Molecular and Cellular Neurosciences·A Espana, F Clotman
Oct 31, 2012·The European Journal of Neuroscience·Elisa J HoekstraMarten P Smidt
Oct 31, 2012·Molecular and Cellular Biology·Lukáš ČajánekErnest Arenas
Jan 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Emma R AnderssonErnest Arenas
Apr 23, 2013·Developmental Biology·Shane V HegartyGerard W O'Keeffe
May 3, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Simon R W StottSiew-Lan Ang
Jul 19, 2013·Development·Jesse V VeenvlietMarten P Smidt
Nov 30, 2013·Journal of Molecular Cell Biology·Milan Joksimovic, Rajeshwar Awatramani

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Citations

Feb 23, 2017·Scientific Reports·Stéphanie Le GrasKarine Merienne
Jun 13, 2017·Frontiers in Molecular Neuroscience·Karolina U KabayizaFrédéric Clotman
Jul 8, 2020·International Journal of Molecular Sciences·Simone Mesman, Marten P Smidt
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Quirino Attilio VassalliAnnamaria Locascio

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