PMID: 5575Apr 1, 1976

Interaction between phloretin and the red blood cell membrane

The Journal of General Physiology
M L Jennings, A K Solomon

Abstract

Phloretin binding to red blood cell components has been characterized at pH6, where binding and inhibitory potency are maximal. Binding to intact red cells and to purified hemoglobin are nonsaturated processes approximately equal in magnitude, which strongly suggests that most of the red cell binding may be ascribed to hemoglobin. This conclusion is supported by the fact that homoglobin-free red cell ghosts can bind only 10% as much phloretin as an equivalent number of red cells. The permeability of the red cell membrane to phloretin has been determined by a direct measurement at the time-course of the phloretin uptake. At a 2% hematocrit, the half time for phloretin uptake is 8.7s, corresponding to a permeability coefficient of 2 x 10(-4) cm/s. The concentration dependence of the binding to ghosts reveals two saturable components. Phloretin binds with high affinity (K diss = 1.5 muM) to about 2.5 x 10(6) sites per cell; it also binds with lower affinity (Kdiss = 54 muM) to a second (5.5 x 10(7) per cell) set of sites. In sonicated total lipid extracts of red cell ghosts, phloretin binding consists of a single, saturable component. Its affinity and total number of sites are not significantly different from those of the low affi...Continue Reading

References

Jan 1, 1976·The Journal of General Physiology·M PoznanskyA K Solomon
Jan 1, 1974·The Journal of Membrane Biology·J D OwenE M Eyring
Dec 1, 1972·Biochimica Et Biophysica Acta·J D Owen, A K Solomon
Jun 1, 1973·The Journal of Clinical Investigation·S LevineR M Hays
Nov 16, 1973·Biochimica Et Biophysica Acta·M P CzechW S Lynn
Jan 17, 1972·Biochimica Et Biophysica Acta·P Seeman, S Roth
Feb 1, 1972·Archives of Internal Medicine·G Guidotti
Jan 1, 1972·The Journal of Membrane Biology·I BenesA Kotyk
Aug 3, 1971·Biochemistry·A KahlenbergD Dolansky
Jul 7, 1970·Biochimica Et Biophysica Acta·R I Macey, R E Farmer
Sep 20, 1968·Archives of Biochemistry and Biophysics·D F Diedrich
Jan 1, 1969·Journal of Theoretical Biology·C T EverittD A Haydon
Jul 1, 1967·The Journal of General Physiology·A Cass, A Finkelstein
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Oct 8, 1962·Biochimica Et Biophysica Acta·J P HUMMEL, W J DREYER
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN
Jan 1, 1965·Journal of the American Oil Chemists' Society·W S SINGLETONJ L WHITE

Citations

Apr 1, 1993·Pflügers Archiv : European journal of physiology·S MartialP Ripoche
Aug 26, 1976·The Journal of Membrane Biology·M L Jennings
Jan 1, 1987·The Journal of Membrane Biology·M R Toon, A K Solomon
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·W A Murphy, R D Lumsden
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·R D Lumsden, W A Murphy
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·E TetaudT Baltz
Jun 1, 1980·The Journal of General Physiology·A S Verkman, A K Solomon
May 1, 1982·The Journal of General Physiology·J Brahm
Sep 16, 2008·Applied and Environmental Microbiology·E P BriczinskiR F Roberts
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·R E ForsterM Wunder
Jun 1, 1980·Journal of Neurochemistry·R P SanetoJ R Perez-Polo
Jan 1, 1983·Annals of the New York Academy of Sciences·A K SolomonA S Verkman
Feb 13, 2001·Biophysical Journal·J I Alakoskela, P K Kinnunen
Jan 14, 2017·Scientific Reports·Jurgen R HaanstraB M Bakker
Nov 11, 1980·Biochemistry·D C Sogin, P C Hinkle
Sep 1, 1989·Archives of Biochemistry and Biophysics·J PeinadoT P Akerboom
Jan 1, 1995·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J P Cartron, P Ripoche
Mar 1, 1977·The Journal of Experimental Zoology·R M HaysH Berliner
Apr 4, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T M RodelaP A Wright
May 9, 1998·The Journal of Biological Chemistry·N RoudierF Tacnet
Apr 17, 2008·Molecular and Cellular Biochemistry·Yury S TarahovskyYuri A Kim
Feb 1, 1987·The American Journal of Physiology·O Fröhlich, R B Gunn

Related Concepts

Plasma Membrane
Cell Membrane Permeability
Erythrocytes
Glucose, (beta-D)-Isomer
Eryhem
Hemolysis
Hydrogen-Ion Concentration
Phloretin
Lipid Metabolism

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