Interaction between rose bengal and different protein components

Cornea
S C Tseng, S H Zhang

Abstract

Bindings of rose bengal to several proteins were determined by Sephadex G-75 chromatography. Their respective blocking effect against dye uptake was demonstrated in an assay using a rabbit corneal epithelial cell layer. The total binding capacity of nonmucin proteins was measured using fluorometry and Scatchard analysis. The results showed that albumin, lactoferrin, transferrin, and lysozyme could--but serum prealbumin, IgA, carboxymethyl cellulose (CMC), and Sepharose 4B-purified porcine stomach mucin (PSM) could not--bind rose bengal. Lysozyme formed precipitates with rose bengal. Sufficient concentrations of albumin, lactoferrin, transferrin, or lysozyme premixed with rose bengal could block dye uptake by cells, but IgA and serum prealbumin could not. Premixed PSM was not as effective as precoated PSM in blocking dye uptake. The dissociation constant (Kd) was 1.2 x 10(-7) M, 3.6 x 10(-7) M, 3.9 x 10(-7) M, and 1.6 x 10(-6) M for albumin, transferrin, lactoferrin, and lysozyme, respectively. Based on these values, the total maximal binding capacity of nonmucin proteins in normal 7-microliters tears was extrapolated to be 0.249 micrograms rose bengal, which is too small to explain the negative staining of rose bengal on the no...Continue Reading

Citations

Sep 26, 2003·The British Journal of Ophthalmology·S ShimmuraK Tsubota
Apr 26, 2008·Investigative Ophthalmology & Visual Science·Timothy D BlalockIlene K Gipson
Dec 24, 1997·American Journal of Ophthalmology·S H Lee, S C Tseng
Apr 22, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Liang JiaDavid L Kaplan
Oct 12, 2012·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Nathan Efron
Feb 1, 2003·Lasers in Surgery and Medicine·Eric WachterAmy Cada
Jun 13, 2015·Journal of Ophthalmology·Lasse Jørgensen CehofskiHenrik Vorum
Dec 14, 2004·Current Eye Research·Doreen Khan-Lim, Monica Berry
Dec 18, 2019·Essays in Biochemistry·Eduardo Fuentes-Lemus, Camilo López-Alarcón
Oct 27, 1998·Current Eye Research·T SitaramammaG N Rao
Aug 26, 2019·Free Radical Biology & Medicine·Eduardo Fuentes-LemusMichael J Davies

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