Interaction between the membrane protein of a pathogen and insect microfilament complex determines insect-vector specificity

Proceedings of the National Academy of Sciences of the United States of America
Shiho SuzukiS Namba

Abstract

Many insect-transmissible pathogens are transmitted by specific insect species and not by others, even if they are closely related. The molecular mechanisms underlying such strict pathogen-insect specificity are poorly understood. Candidatus Phytoplasma asteris, OY strain, line W (OY), is a phytopathogenic bacterium transmitted from plant to plant by sap-feeding insect vectors (leafhoppers). Our study focused on an abundant cell-surface membrane protein of the phytoplasma named antigenic membrane protein (Amp), which is not homologous with any reported functional protein. Immunofluorescence microscopy of the phytoplasma-infected insect showed that OY phytoplasma was localized to the microfilaments of the visceral smooth muscle surrounding the insect's intestinal tract. The affinity column assay showed that Amp forms a complex with three insect proteins: actin, myosin heavy chain, and myosin light chain. Amp-microfilament complexes were detected in all OY-transmitting leafhopper species, but not in the non-OY-transmitting leafhoppers, suggesting that the formation of the Amp-microfilament complex is correlated with the phytoplasma-transmitting capability of leafhoppers. Although several studies have reported interactions between...Continue Reading

References

Aug 23, 1996·Biochemical and Biophysical Research Communications·G M DeLucaD P Blackall
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·S H KappeJ H Adams
Sep 16, 1999·The EMBO Journal·R D Hayward, V Koronakis
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S J JurisJ E Dixon
Oct 6, 2000·Annual Review of Microbiology·I M LeeD E Gundersen-Rindal
Nov 23, 2000·Journal of Virology·Y SuzukiY Kawaoka
May 31, 2001·Science·D PantaloniM F Carlier
Sep 12, 2001·Molecular Plant-microbe Interactions : MPMI·S KakizawaS Namba
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·A K GhoshM Jacobs-Lorena
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·M B Goldberg
Feb 21, 2002·Trends in Cell Biology·Richard D Hayward, Vassilis Koronakis
Aug 16, 2002·Molecular Microbiology·Robin M Delahay, Gad Frankel
Apr 18, 2003·Nature·Samantha Gruenheid, B Brett Finlay
Oct 24, 2008·Phytopathology·I M LeeA Bertaccini

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Citations

Mar 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ayaka HoshiShigetou Namba
Mar 22, 2012·Molecular Plant-microbe Interactions : MPMI·K BoonrodG Krczal
Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Xiaodong BaiSaskia A Hogenhout
Mar 10, 2009·Applied and Environmental Microbiology·Agnès CimermanXavier Foissac
Nov 10, 2013·Molecular Plant-microbe Interactions : MPMI·Nelson A WulffDean W Gabriel
Aug 16, 2011·Annual Review of Phytopathology·Akiko SugioSaskia A Hogenhout
May 3, 2012·TheScientificWorldJournal·Michael KubeErich Seemüller
May 1, 2012·Current Opinion in Microbiology·Akiko Sugio, Saskia A Hogenhout
Jul 22, 2008·Current Opinion in Plant Biology·Saskia A Hogenhout, Rosemary Loria
Apr 23, 2015·International Journal of Systematic and Evolutionary Microbiology·Wei WeiYan Zhao
Aug 19, 2008·Molecular Plant Pathology·Saskia A HogenhoutShigetou Namba
Sep 18, 2010·Journal of Applied Microbiology·S MuroloG Romanazzi
Jan 22, 2013·International Journal of Systematic and Evolutionary Microbiology·Fabio QuaglinoRobert Edward Davis
Dec 10, 2013·Journal of Insect Physiology·Fumiko YukuhiroHiroaki Noda
Feb 11, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sergio Murolo, Gianfranco Romanazzi
Oct 11, 2014·FEMS Microbiology Letters·Yutaro NeriyaShigetou Namba
Feb 19, 2009·FEMS Microbiology Letters·Shigeyuki KakizawaShigetou Namba
May 21, 2009·Phytopathology·Christoph J MayerJürgen Gross
Apr 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiao ZhouJiang-Hua Sun
Jul 8, 2016·Microbiology·Alisa KonnerthKajohn Boonrod

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