PMID: 8595883Feb 1, 1996Paper

Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro

Genes & Development
C S LutzJ C Alwine

Abstract

We have previously shown that the U1 snRNP-A protein (U1A) interacts with elements in SV40 late polyadenylation signal and that this association increases polyadenylation efficiency. It was postulated that this interaction occurs to facilitate protein-protein association between components of the U1 snRNP and proteins of the polyadenylation complex. We have now used GST fusion protein experiments, coimmunoprecipitations and Far Western blot analyses to demonstrate direct binding between U1A and the 160-kD subunit of cleavage-polyadenylation specificity factor (CPSF). In addition, Western blot analyses of fractions from various stages of CPSF purification indicated that U1A copurified with CPSF to a point but could be separated in the highly purified fractions. These data suggest that U1A protein is not an integral component of CPSF but may be able to interact and affect its activity. In this regard, the addition of purified, recombinant U1A to polyadenylation reactions containing CPSF, poly(A) polymerase, and a precleaved RNA substrate resulted in concentration-dependent increases in both the level of polyadenylation and poly(A) tail length. In agreement with the increase in polyadenylation efficiency caused by U1A, recombinant...Continue Reading

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Citations

Aug 31, 2002·Journal of Molecular Recognition : JMR·Carol S LutzJudith A James
May 2, 2013·Trends in Biochemical Sciences·Bin Tian, James L Manley
Mar 6, 2003·Molecular Immunology·Christine MilcarekCarol S Lutz
Sep 6, 2002·Biochimica Et Biophysica Acta·Kenneth James Howe
Jun 17, 1999·FEMS Microbiology Reviews·E Wahle, U Rüegsegger
Apr 26, 2003·Trends in Biochemical Sciences·Hervé Le HirMelissa J Moore
Apr 5, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Axel SchambachChristopher Baum
Jan 25, 2003·Scandinavian Journal of Immunology·O Z Faig, C S Lutz
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H M TragliaA K Hopper
Aug 22, 2002·EMBO Reports·Stefania MillevoiStéphan Vagner
Jun 28, 2011·Genome Biology and Evolution·Eric S Ho, Samuel I Gunderson
Jul 1, 1997·Nucleic Acids Research·G Edwalds-GilbertC Milcarek
Dec 18, 2007·Nucleic Acids Research·Seol-Hoon LeeYounghoon Lee
Jan 2, 2010·Nucleic Acids Research·Stefania Millevoi, Stéphan Vagner
Sep 16, 2006·Journal of Virology·Jeremy E Wilusz, Karen L Beemon
Mar 25, 2000·Molecular and Cellular Biology·H Hans, J C Alwine
Apr 3, 2001·Molecular and Cellular Biology·S A BeckleyD W Rowe
Dec 12, 2001·Molecular and Cellular Biology·Susan McCrackenBenjamin J Blencowe
Oct 17, 2003·RNA·Sita Awasthi, James C Alwine
Dec 11, 2008·PLoS Biology·Francesco Catania, Michael Lynch
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Mark T McNally
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K MartincicC Milcarek

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