PMID: 9551251Apr 29, 1998Paper

Interaction between triiodothyronine and ovarian steroid hormones on the regulation of the release of thyrotropin and thyrotropin-releasing hormone in vitro

The Chinese Journal of Physiology
S R LiuP S Wang

Abstract

In vivo and in vitro experiments were designed to examine [1] the effect of triiodothyronine (T3) and/or ovarian steroids on the spontaneous and thyrotropin-releasing hormone (TRH)-stimulated release of thyrotropin (TSH) by the anterior pituitary gland (AP) in vitro; and [2] the in vivo effects of T3 and ovarian steroids on TRH-release in vitro. In the experiment 1, ovariectomized-thyroidectomized (Ovx-Tx) rats were injected with triiodothyronine (T3, 2 micrograms/kg), estradiol benzoate (EB, 25 micrograms/kg), progesterone (P, 10 mg/kg), T3 plus EB, T3 plus P, EB plus P, or T3 plus EB and P for 6 days before decapitation. The AP was incubated with Locke's medium, challenged with TRH (30 nM), recovered and then with T3 (10 nM) only or with T3+TRH, 30 min for each interval. Mediobasal hypothalami (MBHs) were challenged with high potassium (60 mM) for 30 min. In the experiment 2, the APs of Ovx-Tx rats were enzymatically dispersed and the AP cells were pretreated with or without EB (0-6 nM) for 72 h, and further with T3 (10 nM) for 24 h, followed by an incubation for 30 min with TRH (0-100 nM). The spontaneous and TRH-induced release of TSH in vitro from rat APs, and pituitary TSH content were increased by T3, or T3 plus P as com...Continue Reading

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