Interaction between VEGF and calcium-independent phospholipase A2 in proliferation and migration of retinal pigment epithelium

Current Eye Research
Anne Katrine KehlerMiriam Kolko

Abstract

Inhibition of VEGF in the eye is an important treatment modality for reducing proliferation and migration of retinal pigment epithelium (RPE) in age-related macular degeneration (AMD). Additionally, previous studies suggest calcium-independent phospholipase A(2) group VIA (iPLA(2)-VIA) to be a potential regulator of cell proliferation and migration, and evidence show abundant expression of iPLA(2)-VIA in RPE cells. The aim of the present study was to evaluate the potential role of iPLA(2)-VIA in VEGF-induced proliferation and migration of RPE cells. The human RPE cell line, ARPE-19, was used in all assays. To explore the role of iPLA(2)-VIA in VEGF-induced RPE proliferation and migration, iPLA(2)-VIA inhibition by the iPLA(2)-VIA specific inhibitor, bromoenol lactone, was done. RPE cell proliferation and migration were evaluated by measurements of incorporated radioactive thymidine in DNA and by a Boyden chamber technique, respectively. A luciferase assay monitored the VEGF-induced iPLA(2)-VIA transcriptional activity. Western blot analysis and an activity assay were used to detect the protein levels and activity of iPLA(2)-VIA respectively after treatment with VEGF. RPE cells treated with VEGF showed significant increased prol...Continue Reading

References

Dec 13, 1996·The Journal of Biological Chemistry·M A BalboaE A Dennis
Feb 1, 1997·The British Journal of Ophthalmology·M KliffenP T de Jong
Apr 1, 1997·Current Opinion in Cell Biology·W H MoolenaarG C Zondag
Mar 1, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·T IshibashiH Inomata
Jun 27, 1997·The Journal of Biological Chemistry·J Balsinde, E A Dennis
Feb 5, 1999·Progress in Retinal and Eye Research·P HiscottI Grierson
Jun 24, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·P DevillierC Advenier
Dec 11, 1999·Proceedings of the Association of American Physicians·C O Bingham, K F Austen
Aug 31, 2000·The Journal of Biological Chemistry·A K RoshakL A Marshall
Oct 18, 2002·Journal of Cellular Physiology·Teresa Sanchez, Juan J Moreno
Dec 20, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Reinier O Schlingemann
Apr 23, 2004·Ophthalmic Research·Christoph W SpraulGabriele E Lang
Sep 24, 2004·The Journal of Biological Chemistry·Alex D Manguikian, Suzanne E Barbour
Sep 13, 2006·The Journal of Biological Chemistry·Shane P Herbert, John H Walker
Sep 16, 2006·Biochimica Et Biophysica Acta·Ralph H Schaloske, Edward A Dennis
Feb 13, 2007·The Journal of Biological Chemistry·Moumita GhoshChristina C Leslie
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Miriam KolkoNicolas G Bazan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.