Interaction, cooperative promoter modulation, and renal colocalization of GCMa and Pitx2.

The Journal of Biological Chemistry
Steffen Wolfgang SchubertSaid Hashemolhosseini

Abstract

The transcription factor GCMa is a member of a new small family of transcription factors with a conserved zinc-containing DNA-binding domain. All members of this transcription factor family play crucial roles as master regulators during development. GCMa is restricted to placenta during development and to kidney and thymus at postnatal stages. It is essential for the formation of the placental labyrinth and as a consequence for survival of the embryo from mid-embryogenesis onwards. Here, we identify Pitx transcription factors as GCMa-interacting proteins. We show that Pitx proteins interact via their conserved homeodomain with the DNA-binding domain of GCMa. As a consequence, Pitx proteins and GCMa exhibit cooperative DNA binding. Furthermore, Pitx proteins influence GCMa-dependent promoter activation in a cell-specific manner. One of the three Pitx paralogues in mice, Pitx2, is the predominant Pitx member present in the placenta and colocalizes on the cellular level with GCMa in the kidney. This is the first description of a regulatory cross-talk between a transcription factor of the GCM family and a homeodomain protein.

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Citations

Mar 11, 2006·Nucleic Acids Research·Hsiao-Ching ChuangHungwen Chen
Aug 11, 2006·Journal of Neurochemistry·Tatiana CheusovaSaid Hashemolhosseini
Apr 1, 2006·Genes, Chromosomes & Cancer·Birgit ZirnManfred Gessler
Jan 3, 2008·The Journal of Biological Chemistry·Steffen Wolfgang SchubertSaid Hashemolhosseini
Aug 14, 2019·PloS One·Takahito OhiraHiroyuki Kugoh
Oct 12, 2010·American Journal of Physiology. Renal Physiology·Masanori KugitaShizuko Nagao

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