Interaction forces measured using AFM between colloids and surfaces coated with both dextran and protein

Langmuir : the ACS Journal of Surfaces and Colloids
Li-Chong Xu, Bruce E Logan

Abstract

Both proteins and polysaccharides are biopolymers present on a bacterial surface that can simultaneously affect bacterial adhesion. To better understand how the combined presence of proteins and polysaccharides might influence bacterial attachment, adhesion forces were examined using atomic force microscopy (AFM) between colloids (COOH- or protein-coated) and polymer-coated surfaces (BSA, lysozyme, dextran, BSA+dextran and lysozyme+dextran) as a function of residence time and ionic strength. Protein and dextran were competitively covalently bonded onto glass surfaces, forming a coating that was 22-33% protein and 68-77% dextran. Topographic and phase images of polymer-coated surfaces obtained with tapping mode AFM indicated that proteins at short residence times (<1 s) were shielded by dextran. Adhesion forces measured between colloid and polymer-coated surfaces at short residence times increased in the order protein+dextran < or = protein < dextran. However, the adhesion forces for protein+dextran-coated surface substantially increased with longer residence times, producing the largest adhesion forces between polymer coated surfaces and the colloid over the longest residence times (50-100 s). It was speculated that with longer...Continue Reading

References

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Citations

Sep 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sangjin Ryu, Christian Franck
Mar 12, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Li-Chong Xu, Christopher A Siedlecki
May 1, 2007·Biomaterials·Li-Chong Xu, Christopher A Siedlecki
Jun 10, 2010·Biochimica Et Biophysica Acta·Zhenyu ZhangMark Geoghegan
Aug 5, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yihui DongXiaohua Lu
Sep 20, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Sanjai J Parikh, Jon Chorover
Jan 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Richard GilEmmanuelle Schulz

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