PMID: 2105211Jan 12, 1990Paper

Interaction in situ of the cytoskeletal protein vinculin with bilayers studied by introducing a photoactivatable fatty acid into living chicken embryo fibroblasts

European Journal of Biochemistry
V NiggliM M Burger

Abstract

The cytoskeletal protein vinculin, a putative actin--plasma-membrane linker, has been shown by hydrophobic photo-labeling to interact in vitro directly with bilayers of acidic phospholipids [Niggli et al. (1986) J. Biol. Chem. 261, 6912-6918]. In order to demonstrate that such an interaction occurs also in intact cells, chicken embryo fibroblasts were incubated for 2 h with a 3H-labeled photoactivatable fatty acid, 11-(4-[3-(trifluoromethyl)-diazirinyl]phenyl)-[2-3H]undecanoic acid. This resulted in biosynthetic incorporation into cellular lipids of a fraction of the fatty acid added. Following photolysis, vinculin was immunoprecipitated from different subcellular fractions using a specific polyclonal anti-vinculin antibody. The protein was recovered from both the cytosolic and the crude membrane fraction. Vinculin from both fractions incorporated label, but the membrane-associated population was at least eight times more strongly photolabeled than the cytosolic protein. Moreover, photolysis increased only labeling of the membrane-bound but not of the cytosolic protein. These results suggest that the direct interaction of vinculin with the hydrophobic core of the phospholipid layer observed in vitro may also be relevant in inta...Continue Reading

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Citations

Nov 1, 1992·Journal of Cellular Biochemistry·J Hagmann, M M Burger
Jun 9, 1998·Cellular Signalling·C Keenan, D Kelleher
Oct 9, 2001·Trends in Biochemical Sciences·V Niggli
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Hagmann
Jul 1, 1993·The Journal of Cell Biology·S J SamuelssonR J Bloch
Mar 30, 2006·Theoretical Biology & Medical Modelling·David L ScottWolfgang H Goldmann
Jun 17, 2008·Biochemical and Biophysical Research Communications·Gerold DiezWolfgang H Goldmann
Mar 16, 2013·Cell Biology International·Wolfgang H GoldmannBen Fabry
Mar 6, 2009·Cell Adhesion & Migration·María Gabriela Márquez, Norma Beatriz Sterin-Speziale
Oct 6, 2016·Biophysical Journal·Mridula Dwivedi, Roland Winter
Jan 21, 1993·Nature·V Niggli
Sep 30, 1999·Molecular Membrane Biology·J E Johnson, R B Cornell

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