Interaction map of Arabidopsis Mediator complex expounding its topology

Nucleic Acids Research
Sourobh MajiJitendra K Thakur

Abstract

Understanding of mechanistic details of Mediator functioning in plants is impeded as the knowledge of subunit organization and structure is lacking. In this study, an interaction map of Arabidopsis Mediator complex was analyzed to understand the arrangement of the subunits in the core part of the complex. Combining this interaction map with homology-based modeling, probable structural topology of core part of the Arabidopsis Mediator complex was deduced. Though the overall topology of the complex was similar to that of yeast, several differences were observed. Many interactions discovered in this study are not yet reported in other systems. AtMed14 and AtMed17 emerged as the key component providing important scaffold for the whole complex. AtMed6 and AtMed10 were found to be important for linking head with middle and middle with tail, respectively. Some Mediator subunits were found to form homodimers and some were found to possess transactivation property. Subcellular localization suggested that many of the Mediator subunits might have functions beyond the process of transcription. Overall, this study reveals role of individual subunits in the organization of the core complex, which can be an important resource for understandin...Continue Reading

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Citations

Apr 25, 2020·International Journal of Molecular Sciences·Lingjie Zhang, Changkui Guo
Dec 1, 2020·The New Phytologist·Naohiko OhamaNam-Hai Chua
Dec 17, 2020·Journal of Integrative Plant Biology·Jing GuoXin-Jian He

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Methods Mentioned

BETA
two-hybrid
co-immunoprecipitation
pull-down
immunoprecipitation
two hybrid
Fluorescence
Y2H

Software Mentioned

SWISS
cpptraj
Phyre2 server
web
IntAct
AMBER16 package
ClustalW
MODEL
MOTIF Search
PROCHECK

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