Interaction network of tobacco etch potyvirus NIa protein with the host proteome during infection

BMC Genomics
Fernando MartínezJosé-Antonio Daròs

Abstract

The genomes of plant viruses have limited coding capacity, and to complete their infectious cycles, viral factors must target, direct or indirectly, many host elements. However, the interaction networks between viruses and host factors are poorly understood. The genus Potyvirus is the largest group of plus-strand RNA viruses infecting plants. Potyviral nuclear inclusion a (NIa) plays many roles during infection. NIa is a polyprotein consisting of two domains, viral protein genome-linked (VPg) and protease (NIaPro), separated by an inefficiently utilized self-proteolytic site. To gain insights about the interaction between potyviral NIa and the host cell during infection, we constructed Tobacco etch virus (TEV, genus Potyvirus) infectious clones in which the VPg or the NIaPro domains of NIa were tagged with the affinity polypeptide Twin-Strep-tag and identified the host proteins targeted by the viral proteins by affinity purification followed by mass spectrometry analysis (AP-MS). We identified 232 different Arabidopsis thaliana proteins forming part of complexes in which TEV NIa products were also involved. VPg and NIaPro specifically targeted 89 and 76 of these proteins, respectively, whereas 67 proteins were targeted by both ...Continue Reading

References

Jun 1, 1992·The Plant Cell·S UknesJ Ryals
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Nov 14, 1997·Virology·J A Daròs, J C Carrington
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Jan 22, 2004·Nature Reviews. Genetics·Albert-László Barabási, Zoltán N Oltvai
Jun 26, 2004·The Journal of Biological Chemistry·Pietri Puustinen, Kristiina Mäkinen
Dec 14, 2005·Trends in Plant Science·Christophe Robaglia, Carole Caranta
Aug 1, 1987·Journal of Virology·J C Carrington, W G Dougherty
Jun 28, 2007·Nature Reviews. Molecular Cell Biology·Anne-Claude GingrasRuedi Aebersold
Jan 10, 2008·The Plant Journal : for Cell and Molecular Biology·Carine CharronCarole Caranta
Jun 12, 2008·PloS One·Patricia Agudelo-RomeroSantiago F Elena
Aug 30, 2008·The Journal of General Virology·Philippe J DufresneJean-François Laliberté
Nov 6, 2008·Molecular Systems Biology·B de ChasseyV Lotteau
Jun 9, 2009·PLoS Computational Biology·Patrycja Vasilyev MissiuroHui Ge
Jul 25, 2009·The Plant Journal : for Cell and Molecular Biology·Xiaohua YangShunyuan Xiao
Feb 16, 2010·Virus Research·Leonor C Bedoya, José-Antonio Daròs
May 4, 2010·Nucleic Acids Research·Zhou DuZhen Su
Jul 24, 2010·Nature Reviews. Molecular Cell Biology·Harm H Kampinga, Elizabeth A Craig
Jun 24, 2011·Journal of Virology·Katri EskelinKristiina Mäkinen
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Jul 30, 2011·Science·UNKNOWN Arabidopsis Interactome Mapping Consortium
May 9, 2012·Annual Review of Cell and Developmental Biology·Corné M J PieterseSaskia C M Van Wees
May 23, 2012·Proteomics·Wade H DunhamAnne-Claude Gingras
Sep 29, 2012·Current Opinion in Virology·Santiago F Elena, Guillermo Rodrigo

❮ Previous
Next ❯

Citations

Jun 14, 2017·Molecular Plant-microbe Interactions : MPMI·Geneviève Tavert-RoudetThierry Michon
Oct 14, 2017·Molecular Biology and Evolution·Justine CharonThierry Michon
Jun 6, 2018·Frontiers in Plant Science·Bernardo RodamilansJuan Antonio García
Aug 8, 2020·Frontiers in Plant Science·Francesca MaioHarrold A van den Burg
Jan 18, 2019·Viruses·Krin S Mann, Hélène Sanfaçon
Oct 6, 2020·3 Biotech·Sankara Naynar PalaniJebasingh Tennyson
Mar 1, 2018·3 Biotech·Hamid Reza MaghamniaAbdolbaset Azizi
Jul 30, 2020·Viruses·Maathavi KannanHamidun Bunawan
Feb 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Rubén GonzálezSantiago F Elena
Feb 19, 2021·Archives of Virology·Sankara Naynar PalaniJebasingh Tennyson
May 1, 2021·Microorganisms·Sandra Martínez-TuriñoJuan Antonio García
Jun 29, 2021·PLoS Computational Biology·Silvia AmbrósGuillermo Rodrigo
Sep 14, 2018·ACS Synthetic Biology·Teresa CorderoJosé-Antonio Daròs
Apr 9, 2019·Current Opinion in Plant Biology·Kristiina Mäkinen, Swarnalok De

❮ Previous
Next ❯

Datasets Mentioned

BETA
L08752

Methods Mentioned

BETA
affinity purification
PCR
electrophoresis
two-hybrid

Software Mentioned

ProteinPilot
Cytoscape
BLAST
agriGO
Mascot
REVIGO
ProteinPilot Progroup
Panther

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.