Interaction of a 3' RNA region of the mustard trnK gene with chloroplast proteins.

Nucleic Acids Research
J Nickelsen, G Link

Abstract

The 3' flanking region of the chloroplast trnK gene for tRNALys of mustard contains a palindromic sequence previously implicated with transcription termination and/or processing of the precursor RNA. Here we have investigated whether RNA sequences from the trnK 3' region are capable of interacting with chloroplast proteins in vitro. We find specific binding to an RNA region which is located further downstream from the palindromic sequence. The approximate length and position of this 3' binding region is reflected by a 41 nt spanning RNA segment which is protected against RNase T1 digestion by chloroplast protein(s). Competition experiments and sequence analyses suggest that U residues play an essential role in the RNA-protein interaction. Only a small number of proteins, possibly one single species, is in contact with the trnK 3' RNA.

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Citations

Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Feb 1, 1992·Molecular & General Genetics : MGG·C MonodJ D Rochaix
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M L SalvadorL Bogorad
May 14, 2005·Antioxidants & Redox Signaling·Thomas Pfannschmidt, Karsten Liere
Jun 11, 1991·Nucleic Acids Research·Y Q Li, M Sugiura
May 1, 1992·Plant Molecular Biology·M Sugiura

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