Interaction of a 43-kDa receptor-like protein with a 4-kDa hormone-like peptide in soybean

Biochemistry
Kazuki Hanada, H Hirano

Abstract

A 43-kDa soybean protein is a receptor-like protein kinase that is capable of interaction with a 4-kDa hormone-like peptide (leginsulin). The 43-kDa protein consists of alpha and beta subunits; the beta subunit has protein kinase activity that is stimulated by the binding of the 4-kDa peptide. The protein kinase activity is believed to be an early step in a signal transduction cascade, triggered by the peptide. Animal insulin also interacts with the 43-kDa protein and stimulates the protein kinase activity, suggesting that the 4-kDa peptide and insulin bind to the 43-kDa protein with similar mechanisms. To determine the mechanism of interaction between the 4-kDa peptide and 43-kDa protein, we investigated the binding region of the 4-kDa peptide on the 43-kDa protein using surface plasmon resonance (SPR) spectroscopy. We found that the N- (amino acids 1-43) and C-terminal (amino acids 228-251) regions of the alpha subunit of the 43-kDa protein are involved in the binding. The interactions of both insulin and the 4-kDa peptide with the 43-kDa protein were compared using SPR spectroscopy, revealing that insulin binds to the C-terminal regions of the alpha subunit of the 43-kDa protein. These results suggest that the C-terminal reg...Continue Reading

References

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Citations

Jul 8, 2011·The British Journal of Nutrition·Maria Rosa LovatiMarcello Duranti
Nov 21, 2012·Nutrition and Cancer·María del Carmen Robles-RamírezRosalva Mora-Escobedo
Apr 25, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Quentin Kaas, David J Craik
Mar 8, 2008·Peptides·Xin-Peng DunZheng-Wang Chen
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Feb 10, 2007·The FEBS Journal·Xin-Peng DunTomas Bergman
Jan 27, 2005·Biochemical and Biophysical Research Communications·Chengwei ShangHisashi Hirano
Oct 30, 2016·Journal of the American Chemical Society·Baiju P KrishnanKana M Sureshan
Feb 2, 2012·Toxins·Frédéric GressentCorinne Royer
Apr 2, 2021·Journal of Proteomics·Hisashi Hirano

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