Interaction of a DNA-threading netropsin-amsacrine combilexin with DNA and chromatin

Biochemistry
C Bourdouxhe-HousiauxC Bailly

Abstract

Combilexins are a group of DNA ligands having a sequence-specific minor groove binding element combined with an intercalating chromophore which stabilizes the DNA complex and can interfere with topoisomerases. In this study, complementary methods of spectroscopy (absorption, circular dichroism, electric linear dichroism) and biochemistry (viscometry, footprinting) have been applied to explore the nature of the complex formed between a new amsacrine-4-carboxamide-netropsin combilexin and DNA or chromatin. Collectively, the structural and kinetic data concur that the conjugate threads through the DNA double helix so as to intercalate its acridine chromophore, leaving the netropsin moiety and the methanesulfonanilino group positioned within the minor and major grooves of the double helix, respectively. The hybrid retains the AT selectivity conferred by the netropsin moiety. The threading-type intercalation process, evidenced by stopped-flow measurements, is affected when the DNA is wrapped around histones. The composite drug can bind to both the DNA linker segments and the nucleosomal cores in chromatin though, unlike its constituents, it antagonizes the salt-induced condensation of chromatin. As far as its mode of binding to DNA ...Continue Reading

References

Dec 1, 1976·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J Waring
Dec 1, 1992·Journal of Molecular Recognition : JMR·C BaillyC Houssier
Feb 1, 1992·Journal of Biomolecular Structure & Dynamics·P HagmarM Kubista
Sep 1, 1989·Journal of Medicinal Chemistry·L H Hurley
Sep 1, 1987·European Journal of Biochemistry·J Portugal, M J Waring
Jul 28, 1968·Journal of Molecular Biology·W Müller, D M Crothers
Jun 15, 1983·Journal of Molecular Biology·G Snounou, A D Malcom
Apr 1, 1984·Journal of Medicinal Chemistry·J FeigonD R Kearns
Apr 1, 1994·Journal of Biomolecular Structure & Dynamics·H TriebelC Zimmer
Sep 1, 1995·Journal of Medicinal Chemistry·A W McConnaughie, T C Jenkins
Feb 1, 1995·Journal of Biomolecular Structure & Dynamics·C Bailly, M J Waring
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·D ClarkG Felsenfeld

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Citations

Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Carolina CarrascoChristian Bailly
May 15, 2002·European Journal of Medicinal Chemistry·Christian HotzelUlf Pindur
Apr 19, 2002·European Journal of Medicinal Chemistry·Brigitte BaldeyrouMartine Demeunynck
Sep 15, 2000·Bioorganic & Medicinal Chemistry Letters·C B Carlson, P A Beal
Apr 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R GillesC Houssier
Mar 1, 2012·Journal of the American Chemical Society·Yang LiuW David Wilson
May 27, 2011·The Journal of Physical Chemistry. B·Minna LiJohanna Andersson
Mar 22, 2014·Organic & Biomolecular Chemistry·Carmela SpataforaCorrado Tringali
Jan 14, 2016·Molecular BioSystems·K V DileepC Remya
Oct 29, 2010·Bioorganic & Medicinal Chemistry Letters·Lesley A HowellMark Searcey
Nov 13, 2015·International Journal of Biological Macromolecules·Animesh PatraRajib Kumar Mitra
Apr 12, 2013·Chemistry : a European Journal·Johan R JohanssonJohanna Andersson
Jan 31, 2015·Organic & Biomolecular Chemistry·Harmanpreet Kaur PanesarThomas G Minehan
Apr 12, 2016·Journal of Biomolecular Structure & Dynamics·Debipreeta Bhowmik, Gopinatha Suresh Kumar
Sep 17, 2008·Bioorganic & Medicinal Chemistry Letters·Shuhei ImotoFumi Nagatsugi
Jan 11, 2019·Mini Reviews in Medicinal Chemistry·Karolina ArciszewskaDanuta Drozdowska
Nov 16, 2018·MedChemComm·Parteek Prasher, Mousmee Sharma
Aug 26, 2004·Journal of the American Chemical Society·Barry D Gooch, Peter A Beal
Jul 18, 2001·Journal of the American Chemical Society·B OnfeltB Nordén
Sep 20, 2002·Journal of Medicinal Chemistry·Kamesh RastogiTsann-Long Su
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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