Interaction of a plant pseudo-response regulator with a calmodulin-like protein

Biochemical and Biophysical Research Communications
Alexandre PerochonBenoît Ranty

Abstract

Calmodulin (CaM) plays a crucial role in the regulation of diverse cellular processes by modulating the activities of numerous target proteins. Plants possess an extended CaM family including numerous CaM-like proteins (CMLs), most of which appear to be unique to plants. We previously demonstrated a role for CML9 in abiotic stress tolerance and seed germination in Arabidopsis thaliana. We report here the isolation of PRR2, a pseudo-response regulator as a CML9 interacting protein by screening an expression library prepared from Arabidopsis seedlings with CML9 as bait in a yeast two-hybrid system. PRR2 is similar to the response regulators of the two-component system, but lacks the invariant residue required for phosphorylation by which response regulators switch their output response, suggesting the existence of alternative regulatory mechanisms. PRR2 was found to bind CML9 and closely related CMLs but not a canonical CaM. Mapping analyses indicate that an almost complete form of PRR2 is required for interaction with CML9, suggesting a recognition mode different from the classical CaM-target peptide complex. PRR2 contains several features that are typical of transcription factors, including a GARP DNA recognition domain, a Pro-...Continue Reading

References

Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Jun 18, 2002·Plant Physiology·Ildoo HwangJen Sheen
Sep 11, 2002·The Plant Journal : for Cell and Molecular Biology·David W FitterJane A Langdale
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Elian PerrucBenoît Ranty
Jul 28, 2004·Phytochemistry·Vaka S Reddy, Anireddy S N Reddy
Sep 21, 2004·Annual Review of Plant Biology·Alistair M Hetherington, Colin Brownlee
Mar 16, 2005·Plant & Cell Physiology·Takeshi Mizuno, Norihito Nakamichi
May 3, 2005·Annual Review of Plant Biology·Nicolas BouchéHillel Fromm
Aug 3, 2005·The Plant Cell·Peter K Hepler
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·Takeshi Mizuno
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sorina C PopescuS P Dinesh-Kumar
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Fabienne MagnanDidier Aldon
May 1, 2006·Plant Signaling & Behavior·Benoît RantyJean-Philippe Galaud

❮ Previous
Next ❯

Citations

Jul 30, 2011·Biochimie·Alexandre PerochonBenoit Ranty
Mar 5, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hanqiang LiuZhihui Cheng
May 9, 2012·The Plant Journal : for Cell and Molecular Biology·Louis-Jérôme LebaDidier Aldon
Mar 26, 2016·Frontiers in Plant Science·Benoît RantyChristian Mazars
Feb 6, 2013·Biochimica Et Biophysica Acta·Cécilia ChevalBenoît Ranty
Nov 12, 2016·Plant & Cell Physiology·Xiaoyang ZhuJean-Philippe Galaud
Mar 3, 2018·International Journal of Molecular Sciences·Didier AldonJean-Philippe Galaud
May 5, 2017·Plant Signaling & Behavior·Xiaoyang ZhuJean-Philippe Galaud
Mar 2, 2019·Plant Biotechnology Journal·Alexandre PerochonFiona M Doohan
May 2, 2018·International Journal of Molecular Sciences·Valentina La VerdeAlessandra Astegno
Feb 24, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Soo Bin LeeJe Min Lee
Oct 24, 2020·Journal of Experimental Botany·Sujit Jung KarkiAngela Feechan
Aug 17, 2019·Journal of Inorganic Biochemistry·Matteo TrandeAlessandra Astegno
Nov 17, 2020·Journal of Plant Physiology·Tiantian XiongXiaoyang Zhu
Sep 5, 2019·Plant Science : an International Journal of Experimental Plant Biology·M PerezD Aldon
Aug 3, 2013·Plant Physiology·Kyle W Bender, Wayne A Snedden
Nov 9, 2021·Frontiers in Plant Science·Junsub LimSung Chul Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.