Interaction of ABC transport proteins with toxic metals at the level of gene and transport activity in the PLHC-1 fish cell line

Chemico-biological Interactions
C Della TorreI Corsi

Abstract

The aim of this study was to investigate the interaction of four toxic metals with ABC transport proteins in piscine cell line PLHC-1. Cells were exposed for 24 h to 0.01-1 μM of CdCl(2), HgCl(2), As(2)O(3), or K(2)Cr(2)O(7) and the expression of a series of ABC genes (abcb1, abcc1-4) was determined using qRT-PCR. Using the fluorescent model substrates calcein-AM and monochlorbimane we measured interaction of metals with the transport activity of ABC transporters. P-glycoprotein (P-gp) activity was measured in PLHC-1/dox (P-gp overexpressing cells) while activity and interactions of metals with MRPs was measured in PLHC-1/wt cells. After 24 h exposure, abcc2-4 genes were dose-dependently up-regulated by all metals, while abcb1 and abcc1 were less affected. Up-regulation of abcc2 was more pronounced, with up to 8-fold increase in expression. Abcc3 and abcc4 were moderately inducible by HgCl(2) with 3.3-fold and 2.2-fold, respectively. All metals caused a significant inhibition of both P-gp (2.9- to 4-fold vs. controls) and MRP (1.3- to 1.8-fold) transport activities. Modulation of ABC genes and transport activities was further investigated in PLHC-1/wt cells exposed to 1 μM HgCl(2) for 72 h and in Hg resistant cells selected by ...Continue Reading

References

Jan 1, 1992·Critical Reviews in Toxicology·B Kurelec
Dec 1, 1988·The Journal of Experimental Zoology·L E Hightower, J L Renfro
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·T LitmanS E Bates
Apr 19, 2002·The Journal of Pharmacology and Experimental Therapeutics·Sylvie A TerlouwDavid S Miller
Dec 4, 2003·Mutation Research·Gabriel Keith Harris, Xianglin Shi
Apr 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Céline Huynh-DelermeMartine Kolf-Clauw
Apr 23, 2005·Toxicology and Applied Pharmacology·Elaine M LeslieSusan P C Cole
Nov 7, 2006·Biochemical and Biophysical Research Communications·Silvia Franzellitti, Elena Fabbri
Feb 28, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·David S MillerBruce A Stanton
Aug 3, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jonathan M MaherCurtis D Klaassen
May 23, 2008·Archives of Toxicology·Detmar Beyersmann, Andrea Hartwig
Jul 2, 2008·Environmental Science & Technology·David EpelTvrtko Smital
Apr 18, 2009·Toxicology and Applied Pharmacology·Frank Thévenod
Nov 21, 2009·Environmental Science & Technology·Ivana BosnjakAmro Hamdoun
Dec 19, 2009·Archives of Toxicology·Zuzana DrobnáMiroslav Stýblo
Mar 6, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Frank Thévenod
Mar 9, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Wen-Xiong Wang, Philip S Rainbow
Mar 18, 2010·Drug Metabolism Reviews·Xinsheng Gu, Jose E Manautou
May 27, 2010·Archives of Toxicology·Ingrid L Druwe, Richard R Vaillancourt
Nov 30, 2010·Ecotoxicology and Environmental Safety·Juliane Ventura-LimaJosé M Monserrat
Dec 8, 2010·Toxicology and Applied Pharmacology·Christy C BridgesRudolfs K Zalups
Dec 17, 2010·Ecotoxicology and Environmental Safety·Tvrtko SmitalMarijan Ahel
Jan 27, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yong LongZongbin Cui
May 26, 2011·Environmental Science and Pollution Research International·Jie LiuYi-Nian Zhu
Jun 8, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Della TorreI Corsi
Jul 1, 2008·Environmental Toxicology and Pharmacology·Heleen M WortelboerNicole H P Cnubben

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Citations

Jun 5, 2013·Ecotoxicology and Environmental Safety·Camilla Della TorreIlaria Corsi
Aug 5, 2014·Toxicology and Applied Pharmacology·Marta PopovicTvrtko Smital
Aug 28, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bo ShiCaihuan Ke
Oct 14, 2016·Journal of Applied Toxicology : JAT·Adélaïde LereboursJean-Paul Bourdineaud
Aug 4, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Maria Luisa VannucciniIlaria Corsi
May 27, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Till LuckenbachArmin Sturm
May 11, 2016·Environmental Science & Technology·Chun-Yu LaiHe-Ping Zhao
Jul 5, 2016·The Science of the Total Environment·Anastasia GeorgantzopoulouAlberTinka J Murk
Nov 1, 2016·Bulletin of Environmental Contamination and Toxicology·V A AnjosM M Souza
Jul 14, 2019·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Julio C PainefilúCarlos M Luquet
Jul 26, 2018·Marine Pollution Bulletin·Patricia MorcilloAlberto Cuesta

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