Abstract
The interaction of low molecular weight alcohols with low density lipoprotein (LDL) has been studied using amide I band-fitting, thermal profiling and two-dimensional infrared correlation spectroscopy (2D-IR). At 0.3 M alcohol, no changes in secondary structure are observed. In the presence of 1 M alcohol, ethanol and propanol decreases protein denaturation temperature and produces changes in the amide I thermal profiles of protein components and in the lipid bands. The 2D-IR synchronous map corresponding to protein or lipid component at 20-37 degrees C suggests differences in the presence of propanol. The asynchronous map corresponding to the lipid component indicates changes in bandwidth, compatible with a more fluid environment. In the 37-80 degrees C temperature range the thermal profile is different in the presence of propanol, both for the lipid and protein components. The results presented show that when alcohols affect the protein component, the lipid spectrum also varies pointing to an effect on the lipid-protein interaction.
References
Oct 5, 1992·Biochimica Et Biophysica Acta·J S ChiouI Ueda
May 4, 1990·Science·J S ChiouI Ueda
Nov 1, 1987·Chemistry and Physics of Lipids·G JürgensH Esterbauer
Apr 21, 1995·The Journal of Biological Chemistry·S BañuelosG Pifat
Jan 1, 1993·Progress in Biophysics and Molecular Biology·J L ArrondoF M Goñi
Jun 15, 1993·Biochemistry·E GoormaghtighJ M Ruysschaert
Apr 8, 1996·FEBS Letters·J L ArrondoF M Goñi
Jul 14, 1997·Chemistry and Physics of Lipids·M KvederH Esterbauer
Jun 23, 1998·Quarterly Reviews of Biophysics·L K Tamm, S A Tatulian
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·H FabianC P Schultz
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Aug 30, 2000·Alcohol·M KvederM Schara
Mar 10, 2001·Biopolymers·C ViganoJ M Ruysschaert
Aug 2, 2001·Biochemistry·B Pastrana-Rios
Sep 27, 2002·Biophysical Journal·Javier TurnayM Antonia Lizarbe
Mar 8, 2003·Quarterly Reviews of Biophysics·Andreas Barth, Christian Zscherp
Feb 26, 2004·Biochemistry·Alejandro TorrecillasJuan C Gómez-Fernández
Mar 30, 2004·Biopolymers·Thierry LefèvreMichel Pézolet
Jun 11, 2004·Biophysical Journal·Ibon IloroJosé-Luis R Arrondo