Abstract
The interaction of alpha-melanocyte stimulating hormone (alpha-MSH) with negatively charged binary membrane systems composed of either 1,2-dimyristoyl-sn-glycero-3-phosphocholine/1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)], (DMPC/DMPG) or DMPC/1,2-dimyristoyl-sn-glycero-3-phosphate (DMPC/DMPA), both at a 3:1 ratio, was studied using complementary techniques (differential scanning calorimetry, infrared and ultraviolet absorption spectroscopy, and steady-state and time-resolved fluorescence). The peptide structure in buffer, at medium to high concentrations, is a mixture of aggregated beta-strands and random coil, and upon increasing the temperature the random coil configuration becomes predominant. At low concentrations (micromolar) there are essentially no aggregates. When in interaction with the lipidic systems this transition is prevented and the peptide is stabilized in a specific conformation different from the one in solution. The incorporation of alpha-MSH into phosphatidic acid-containing systems produced a significant alteration of the calorimetric data. Lateral heterogeneity can be induced by the peptide in the DMPA-containing mixture, at variance with the one of DMPG. In addition, the lipid/water partition...Continue Reading
References
May 1, 1977·Photochemistry and Photobiology·B Valeur, G Weber
Sep 22, 1992·Biochemistry·K J Willis, A G Szabo
Sep 30, 1991·Biochimica Et Biophysica Acta·A I de KroonB de Kruijff
Mar 12, 1991·Biochemistry·A MugaW K Surewicz
Jan 1, 1991·European Journal of Biochemistry·E ThiaudièreJ Dufourcq
Dec 1, 1989·Journal of Medicinal Chemistry·F Al-ObeidiV J Hruby
Nov 1, 1987·Journal of Medicinal Chemistry·V J HrubyM F Hintz
Dec 3, 1985·Biochemistry·I GrahamJ R Silvius
Feb 1, 1971·Analytical Biochemistry·C W McClare
Jan 1, 1972·Progress in Biophysics and Molecular Biology·A A Zamyatnin
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T K SawyerM E Hadley
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T K SawyerM E Hadley
Nov 16, 1993·Biochemistry·A S ItoA G Szabo
Aug 1, 1995·Biophysical Journal·T E Dahms, A G Szabo
Jan 1, 1996·European Biophysics Journal : EBJ·M H BiaggiM T Lamy-Freund
Jan 1, 1996·Peptides·M GonzálezG D Fidelio
Mar 7, 1996·Biophysical Chemistry·Z Soares MacêdoA S Ito
Oct 1, 1996·Biophysical Journal·L M LouraM Prieto
May 1, 1997·Biophysical Journal·P GaridelA Blume
Dec 16, 1997·Biophysical Chemistry·M H BiaggiM T Lamy-Freund
Jun 24, 1998·Biochemistry·N C SantosM A Castanho
Aug 25, 1999·European Biophysics Journal : EBJ·P G PascuttiA S Ito
Nov 24, 1999·Biochemistry·R N LewisR N McElhaney
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Jan 10, 1985·Biochimica Et Biophysica Acta·M J HopeP R Cullis
Citations
Feb 25, 2003·Chemistry and Physics of Lipids·Luís M S LouraManuel Prieto
Aug 4, 2009·Molecular and Cellular Endocrinology·G Vauquelin, A Packeu
Oct 1, 2010·British Journal of Pharmacology·Georges Vauquelin, Steven J Charlton
Feb 11, 2005·Journal of Molecular Biology·Rodrigo F M de AlmeidaManuel Prieto
Jun 6, 2003·Biochimica Et Biophysica Acta·Nuno C SantosMiguel A R B Castanho
Jul 23, 2016·Therapeutic Delivery·Catarina Oliveira SilvaCatarina Pinto Reis
Jan 31, 2017·Biochimica Et Biophysica Acta. Biomembranes·Shuya K HuangJan K Rainey
Nov 15, 2017·Scientific Reports·Kyungsoo ShinJan K Rainey
Apr 18, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Miguel ManoMaria C Pedroso de Lima
May 5, 2009·Molecular Membrane Biology·Jaime GuillénJosé Villalaín
Sep 5, 2003·The Journal of Biological Chemistry·Francisco N BarreraJosé L Neira
Apr 3, 2003·The Journal of Biological Chemistry·Wenyi ZhangSteven O Smith
Nov 2, 2005·The Biochemical Journal·Francisco N BarreraJosé L Neira
Apr 6, 2018·Biochimica Et Biophysica Acta. Biomembranes·Bashiyar AlmarwaniAnderson Sunda-Meya
May 21, 2008·The Journal of Physical Chemistry. B·Jaime GuillénJosé Villalaín
Aug 28, 2009·The Journal of Physical Chemistry. B·David N Langelaan, Jan K Rainey
Jun 6, 2017·Biophysical Chemistry·Nsoki PhambuAnderson Sunda-Meya
Jan 10, 2002·Biochimica Et Biophysica Acta·Debora Lima de SouzaFrancis Schuber
Jun 15, 2007·Biochimica Et Biophysica Acta·Angela BernabeuJosé Villalaín
Oct 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Nsoki PhambuAnderson Sunda-Meya
May 9, 2018·Biomacromolecules·Valeria CastellettoJanne Ruokolainen
Dec 29, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Thais A EnokiM Teresa Lamy
Jul 21, 2006·The Journal of Physical Chemistry. B·Filipa AbrunhosaMargarida Bastos
Apr 19, 2006·Biochemistry·F FernandesM Prieto
May 12, 2009·Biochimica Et Biophysica Acta·Pierre JoanneIsabel D Alves