Interaction of alpha-melanocyte stimulating hormone with binary phospholipid membranes: structural changes and relevance of phase behavior

Biophysical Journal
L M ContrerasM Prieto

Abstract

The interaction of alpha-melanocyte stimulating hormone (alpha-MSH) with negatively charged binary membrane systems composed of either 1,2-dimyristoyl-sn-glycero-3-phosphocholine/1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)], (DMPC/DMPG) or DMPC/1,2-dimyristoyl-sn-glycero-3-phosphate (DMPC/DMPA), both at a 3:1 ratio, was studied using complementary techniques (differential scanning calorimetry, infrared and ultraviolet absorption spectroscopy, and steady-state and time-resolved fluorescence). The peptide structure in buffer, at medium to high concentrations, is a mixture of aggregated beta-strands and random coil, and upon increasing the temperature the random coil configuration becomes predominant. At low concentrations (micromolar) there are essentially no aggregates. When in interaction with the lipidic systems this transition is prevented and the peptide is stabilized in a specific conformation different from the one in solution. The incorporation of alpha-MSH into phosphatidic acid-containing systems produced a significant alteration of the calorimetric data. Lateral heterogeneity can be induced by the peptide in the DMPA-containing mixture, at variance with the one of DMPG. In addition, the lipid/water partition...Continue Reading

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Citations

Feb 25, 2003·Chemistry and Physics of Lipids·Luís M S LouraManuel Prieto
Aug 4, 2009·Molecular and Cellular Endocrinology·G Vauquelin, A Packeu
Oct 1, 2010·British Journal of Pharmacology·Georges Vauquelin, Steven J Charlton
Jun 6, 2003·Biochimica Et Biophysica Acta·Nuno C SantosMiguel A R B Castanho
Jul 23, 2016·Therapeutic Delivery·Catarina Oliveira SilvaCatarina Pinto Reis
Jan 31, 2017·Biochimica Et Biophysica Acta. Biomembranes·Shuya K HuangJan K Rainey
Apr 18, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Miguel ManoMaria C Pedroso de Lima
Sep 5, 2003·The Journal of Biological Chemistry·Francisco N BarreraJosé L Neira
Apr 6, 2018·Biochimica Et Biophysica Acta. Biomembranes·Bashiyar AlmarwaniAnderson Sunda-Meya
Aug 28, 2009·The Journal of Physical Chemistry. B·David N Langelaan, Jan K Rainey
Jun 6, 2017·Biophysical Chemistry·Nsoki PhambuAnderson Sunda-Meya
Dec 29, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Thais A EnokiM Teresa Lamy
Jul 21, 2006·The Journal of Physical Chemistry. B·Filipa AbrunhosaMargarida Bastos
May 12, 2009·Biochimica Et Biophysica Acta·Pierre JoanneIsabel D Alves

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