Interaction of androgen receptors with androgen response element in intact cells. Roles of amino- and carboxyl-terminal regions and the ligand.

The Journal of Biological Chemistry
U KarvonenJ J Palvimo

Abstract

Promoter interference assay was employed to examine in intact cells the roles of the functional domains of androgen receptor (AR) and the ligand for specific DNA interactions using a cytomegalovirus-(androgen response element)-chloramphenicol acetyltransferase reporter (pCMV-ARE2-CAT). Native rat and human ARs interfered with pCMV-ARE2-CAT expression in a hormone-dependent fashion. Low steroid-independent interference seemed to occur because of the ligand binding domain (LBD), which was transcriptionally inhibitory also in a heterologous context. AR devoid of LBD (rARDelta641-902) decreased pCMV-ARE2-CAT activity by 50%. The rARDelta46-408 mutant devoid of the NH2-terminal transcription activation region exhibited ligand-dependent promoter interference of a similar magnitude. Ligand and DNA binding-deficient mutants (hARM807R and rARC562G, respectively) did not influence pCMV-ARE2-CAT expression, although hARM807R binds to ARE in vitro. Non-steroidal anti-androgens casodex and hydroxyflutamide antagonized agonist-dependent promoter interference, whereas cyproterone acetate, RU 56187, RU 57073, and RU 59063 were partial agonists/antagonists. Collectively, interaction of ARs with ARE in intact cells does not require the presence ...Continue Reading

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Citations

Jun 8, 2001·Journal of Dermatological Science·K ShibaK Adachi
Sep 3, 1998·Molecular Biology of the Cell·A M MoilanenJ J Palvimo
Aug 15, 2012·Molecular and Cellular Biology·Miia RytinkiJorma J Palvimo
Jun 22, 2002·Molecular and Cellular Biology·Noora KotajaJorma J Palvimo
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·H PoukkaJ J Palvimo
Jan 22, 2011·Cellular and Molecular Life Sciences : CMLS·Miia M RytinkiJorma J Palvimo
Jul 28, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Cara L BenjaminJorge A Piedrahita
Jul 30, 2015·Molecular and Cellular Endocrinology·Marjo MalinenJorma J Palvimo
Nov 27, 2015·Biology of Reproduction·Corey ToocheckWilliam H Walker
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Oct 11, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas Thum, Jürgen Borlak
Nov 21, 2001·The Journal of Biological Chemistry·Christin E PetreKaren E Knudsen
Nov 15, 2001·The Journal of Biological Chemistry·Jerry E ChipukDavid Danielpour
Nov 24, 1999·Molecular and Cellular Biology·C L BevanM G Parker
Dec 24, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·James ThompsonPasi A Koivisto
Oct 12, 2002·The Journal of Biological Chemistry·Zhigang KangJorma J Palvimo
Feb 17, 2019·Nature Communications·Fu-Ping ZhangJorma J Palvimo
Jun 26, 1999·The Journal of Biological Chemistry·H PoukkaO A Jänne
Sep 19, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·James ThompsonJorma J Palvimo

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