Interaction of antithrombin III with surface-immobilized albumin-heparin conjugates

Journal of Biomedical Materials Research
C J van DeldenJ Feijen

Abstract

The interaction between antithrombin III (ATIII) and albumin-heparin conjugates covalently coupled onto carboxylated polystyrene beads either in buffer containing albumin or in plasma was studied using 14C-labeled ATIII. Binding isotherms of ATIII were modeled using a summation of two Langmuir equations. These equations describe the binding of ATIII to two different sets of binding sites, one with a high, the other with a low affinity of ATIII to these sites are 9 x 10(6) L/mol and 0.3 x 10(6) L/mol, respectively. The binding of ATIII to surface binding sites with a high affinity for ATIII was correlated with the presence of specific ATIII binding sites in the immobilized heparin. Binding of ATIII from albumin solutions to binding sites with a low affinity for ATIII was dominated by nonspecific binding of ATIII to the immobilized heparin. A third small fraction of the surface bound. ATIII is probably adsorbed to sites on the surface not covered with heparin. In the case of the binding of ATIII to the heparinized surface from plasma solutions, a fraction of initially adsorbed ATIII was desorbed by other plasma proteins. This desorption in combination with direct competition between ATIII and other plasma proteins resulted in low...Continue Reading

References

Nov 1, 1988·Journal of Biomedical Materials Research·K D ParkS W Kim
Jun 1, 1988·Journal of Biomedical Materials Research·W L ChandlerG Schmer
Mar 1, 1969·Journal of Biomedical Materials Research·L Vroman, A L Adams
Oct 1, 1984·Journal of Biomedical Materials Research·W E HenninkJ Feijen
Jan 1, 1983·Biomaterials, Medical Devices, and Artificial Organs·O LarmP Olsson
Mar 1, 1982·Carbohydrate Research·L ThunbergU Lindahl
Dec 1, 1980·Analytical Biochemistry·P K SmithG T Hermanson
Sep 1, 1980·Journal of Biomedical Materials Research·Y MiuraK Miyamoto

❮ Previous
Next ❯

Citations

Feb 19, 1998·Journal of Biomedical Materials Research·E LaemmelM F Sigot-Luizard
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·C J van DeldenJ Feijen
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·C J van DeldenJ Feijen
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·C J van DeldenJ Feijen
Jun 15, 1997·Journal of Biomedical Materials Research·W L HinrichsJ Feijen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.