PMID: 2496752Jan 24, 1989Paper

Interaction of ApoA-1 and ApoE-3 with triglyceride-phospholipid emulsions containing increasing cholesterol concentrations. Model of triglyceride-rich nascent and remnant lipoproteins

Biochemistry
A Derksen, D M Small

Abstract

The cholesterol content of triglyceride-rich lipoproteins increases during their catabolism in circulation. We therefore studied the binding of the exchangeable apoprotein apoA-1 and apoE-3 to triolein-rich emulsions with increasing cholesterol content. Five emulsion systems containing 83.1-88.8% (w/w) triolein, 9.3-10.1% egg yolk phosphatidylcholine, and 1.1-7.3% cholesterol were isolated from sonicated lipid mixtures by flotation. Negative stain EM of emulsions containing 1.1 and 7.3% cholesterol showed polydisperse populations of large spherical particles with diameters of 106 +/- 39 and 108 +/- 57 nm. These values are similar to particle diameters calculated from the lipid composition data. No lamellar structures were observed by EM, even after addition of apoA-1 at a molar ratio to lecithin of 10(-2). Apolipoproteins apoA-1 and apoE-3 bound to the particles in a saturable manner without altering particle morphology. We found a dissociation constant Kd = 7.4 x 10(-7) M and a binding capacity N = 3.9 x 10(-3) proteins/lecithin for apoA-1 with particles containing 1.1% cholesterol; the Kd and N values for apoE-3 were very similar. When the emulsion particles were saturated with cholesterol at 7.3%, the protein binding capacit...Continue Reading

References

Nov 30, 1978·The New England Journal of Medicine·A R Tall, D M Small
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D Atkinson, D M Small
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R J Havel, J P Kane
Feb 26, 1974·Biochemistry·G S David, R A Reisfeld
Jul 1, 1967·The Journal of Cell Biology·R R CardellK R Porter
Mar 1, 1980·The Journal of Clinical Investigation·F ShelburneS Quarfordt

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Citations

Sep 28, 1992·Advances in Colloid and Interface Science·D H Small, M Phillips
Nov 26, 2002·European Journal of Biochemistry·Matthew A PeruginiGeoffrey J Howlett
Nov 5, 2003·FEBS Letters·Heinrich Sandermann
Apr 9, 1998·Biophysical Journal·J B Massey, H J Pownall
Aug 15, 2003·The Journal of Biological Chemistry·Hiroyuki SaitoSissel Lund-Katz

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