PMID: 2105749Feb 6, 1990Paper

Interaction of apolipoprotein A-II with recombinant HDL containing egg phosphatidylcholine, unesterified cholesterol and apolipoprotein A-I

Biochimica Et Biophysica Acta
K A Rye

Abstract

The preparation of discoidal, recombinant HDL (r-HDL) containing various phospholipids, apolipoproteins and a range of concentrations of unesterified cholesterol has been reported by several investigators. The present study describes the preparation of r-HDL containing both apolipoprotein (apo) A-I and apo A-II. r-HDL with 100:1 (mol:mol) egg PC.apo A-I and 0 (Series I), 5 (Series II) or 10 (Series III) mol% unesterified cholesterol were prepared by the cholate dialysis method. The resulting complexes had a Stokes' radius of 4.7 nm and contained two molecules of apo A-I per particle. When the r-HDL (2.0 mg apo A-I) were supplemented with 1.0 mg of apo A-II, one of the apo A-I molecules was replaced by two molecules of apo A-II. This modification was not accompanied by a loss of phospholipid, nor by major change in particle size. The addition of 2.5 or 4.0 mg of apo A-II resulted in the displacement of both apo A-I molecules from a proportion of the r-HDL and the formation of smaller particles (Stokes' radius 3.9 nm), which contained half the original number of egg PC molecules and three molecules of apo A-II. The amount of apo A-I displaced was dependent on the concentration of unesterified cholesterol in the r-HDL: when 2.5 mg...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R PatschS Eisenberg
Nov 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Weisweiler
Jan 1, 1986·Methods in Enzymology·J C Osborne
Aug 28, 2013·Antimicrobial Agents and Chemotherapy·Fabienne BarnaAndrew Hemphill

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Citations

Feb 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·L KritharidesR T Dean
Nov 20, 2004·Clinical Chemistry·Makiko SuzukiMitsuru Seishima
Sep 16, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Donna Lee M DinnesLeonard Kritharides
Jul 26, 1996·The Journal of Biological Chemistry·I C GelissenW Jessup
Jun 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J O Hasler-RapaczJ Rapacz
Nov 1, 1996·The Journal of Biological Chemistry·L KritharidesR T Dean
Sep 17, 1999·The Journal of Biological Chemistry·D ReesL Kritharides

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