PMID: 9418053Jan 7, 1998Paper

Interaction of Arabidopsis kinesin-like calmodulin-binding protein with tubulin subunits: modulation by Ca(2+)-calmodulin

The Plant Journal : for Cell and Molecular Biology
S B NarasimhuluAnireddy S N Reddy

Abstract

Kinesin-like calmodulin-binding protein (KCBP) is a recently identified novel kinesin-like protein that appears to be unique to and ubiquitous in plants. KCBP is distinct from all other known KLPs in having a calmodulin-binding domain adjacent to its motor domain. We have used different regions of KCBP to study its interaction with tubulin subunits and the regulation of this interaction by Ca(2+)-calmodulin. The results show that the carboxy-terminal part of the KCBP, with or without calmodulin-binding domain, binds to tubulin subunits and this binding is sensitive to nucleotides. In the presence of Ca(2+)-calmodulin the motor with calmodulin-binding domain does not bind to tubulin. This Ca(2+)-calmodulin modulation is abolished in the presence of antibodies specific to the calmodulin-binding domain of KCBP. Similar binding studies with the carboxy-terminal part of KCBP lacking the calmodulin-binding domain show no effect of Ca(2+)-calmodulin. These results indicate that Ca(2+)-calmodulin modulates the interaction of KCBP with tubulin subunits and this modulation is due to the calmodulin-binding domain in the KCBP. Calcium-dependent calmodulin modulation of KCBP interaction with tubulin suggests regulation of KCBP function by c...Continue Reading

Citations

Aug 26, 1998·Cell Motility and the Cytoskeleton·B E DeavoursR A Walker
May 31, 2002·Molecular Plant-microbe Interactions : MPMI·Alberto CamasMiguel Lara
Jun 25, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter K HeplerJan W Vos
Jun 14, 2005·Plant Physiology·Salah E Abdel-GhanyAnireddy S N Reddy
Jan 12, 2001·Molecular and Cellular Biology·Z YangL S Goldstein
Feb 2, 2006·BMC Genomics·Dale N RichardsonAnireddy S N Reddy
Mar 4, 2011·Future Microbiology·William Marande, Linda Kohl
Sep 30, 2014·Journal of Experimental Botany·Tania V HumphreyDaphne R Goring
Mar 15, 2006·Trends in Plant Science·Marleen VanstraelenDanny Geelen
Oct 15, 2013·Current Opinion in Plant Biology·Anindya Ganguly, Ram Dixit
Jan 12, 2007·Developmental Biology·Alexander KrichevskyVitaly Citovsky
May 2, 2000·The Journal of Biological Chemistry·I S DayA S Reddy
Jul 1, 2001·The New Phytologist·Wayne A Snedden, Hillel Fromm
Jul 9, 1999·Biochimica Et Biophysica Acta·R Deswal, S K Sopory

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