PMID: 6975384Jan 1, 1981Paper

Interaction of cadmium, calcium, and amiloride in the kinetics of active sodium transport through frog skin

The Japanese Journal of Physiology
M Takada, H Hayashi

Abstract

The epidermal effects of activator (cadmium) or inhibitors (calcium and amiloride) as well as their interactions in active Na transport through bullfrog abdominal skin were analyzed kinetically. Cadmium at 1 mM increased the short circuit (SCC) to 126%. The Hill coefficient (n) for the relation between [Cd2+] and SCC was 0.737, which indicated negative cooperation. Calcium at over 8 mM decreased the SCC to 80% at its maximum effect. Calcium inhibited the Cd2+-induced SCC at lower [Cd2+], and facilitated it at higher [Cd2+]. Calcium acted uncompetitively on the binding reaction of Na+ with a Na entry channel. Cd2+ alone or Cd2+ under the influence of Calcium also acted uncompetitively on the reaction. Amiloride at 5 X 10-5 M inhibited the SCC almost completely. The Hill coefficient in the relation between amiloride and SCC was 0.757, indicating the presence of negative cooperation. In contrast to the effect of calcium, the Cd2+-induced SCC was much reduced at higher [amiloride]. Amiloride inhibited the channel-Na binding reaction in a mixed manner (competitive + noncompetitive), while Cd2+ activated the reaction under the influence of amiloride noncompetitively. From the SCC model based on the hypothesis that the kinetic constan...Continue Reading

Citations

May 1, 1988·Pflügers Archiv : European journal of physiology·W NagelJ Crabbé
Apr 1, 1991·The Journal of Membrane Biology·M M CivanT G O'Brien
Sep 1, 1988·Acta Physiologica Scandinavica·H F Bjerregaard, R Nielsen
Mar 15, 2011·Acta histochemica·Yuko KanekoMakoto Takada
Nov 1, 1985·The Journal of Experimental Zoology·R H Alvarado, T C Cox
Feb 1, 1986·The American Journal of Physiology·S Sariban-Sohraby, D J Benos
May 8, 1995·Biochimica Et Biophysica Acta·V LyallT U Biber
Aug 12, 1982·Biochimica Et Biophysica Acta·C L Thurman, J T Higgins

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