Abstract
CD44 is a glycosylated adhesion molecule which may undergo alternative splicing of 10 possible exons to generate variant isoforms. A number of CD44 variant isoforms expressed by tumor cells have been correlated with metastatic and proliferative behavior. In this study, we have characterized CD44 isoform expression on three prostate cancer cell lines: ALVA-31, PPC-1, and LNCaP. Using reverse transcriptase-polymerase chain reaction, we have found that ALVA-31 and PPC-1 cells express multiple CD44 isoforms, including CD44s (standard form), CD44E (epithelial form), and an exon 14-containing form. In addition, two smaller forms have been detected: one using an alternative donor splice site within exon 5, and a novel form omitting exon 5 entirely. The CD44 isoforms expressed by ALVA-31 and PPC-1 cells appear to be preferentially located on the cell surface. By contrast, LNCaP cells do not express any of the CD44 forms at the RNA or protein level. Both PPC-1 and ALVA-31 cells display tumorigenesis and invasiveness in nude mice, whereas LNCap cells exhibit a less malignant phenotype, suggesting a correlation between CD44 variant (CD44v) expression and aggressive prostate tumor behavior. Functional characterization reveals that CD44 med...Continue Reading
References
Oct 31, 1992·Lancet·Y Matsumura, D Tarin
Apr 22, 1992·JAMA : the Journal of the American Medical Association·H B CarterP C Walsh
Oct 1, 1992·Molecular and Cellular Biology·L Y BourguignonG J Bourguignon
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Jun 29, 1990·Cell·A AruffoB Seed
Apr 5, 1991·Cell·U GünthertP Herrlich
Feb 1, 1992·The Journal of Cell Biology·S Jalkanen, M Jalkanen
Nov 1, 1991·Molecular and Cellular Biology·E Shtivelman, J M Bishop
Apr 1, 1991·The Journal of Cell Biology·T A BrownW G Carter
Jan 1, 1990·Leukemia Research·H TsutaniT Nakamura
Jan 12, 1990·Cell·T St JohnW M Gallatin
Aug 1, 1987·The Journal of Cell Biology·S JalkanenE C Butcher
Mar 24, 1989·Cell·I StamenkovicB Seed
Jul 1, 1989·Experimental Cell Research·D C West, S Kumar
Nov 15, 1989·International Journal of Cancer. Journal International Du Cancer·A R BrothmanD J Merchant
Feb 1, 1988·The Journal of Cell Biology·E L Kalomiris, L Y Bourguignon
Jan 1, 1985·The Prostate·M HasensonA Pousette
Aug 1, 1994·The Journal of Cell Biology·V B LokeshwarL Y Bourguignon
Jan 1, 1995·Journal of Cellular Physiology·N Iida, L Y Bourguignon
Jan 1, 1993·The Prostate·S M LoopR C Ostenson
Oct 23, 1993·Lancet·B MayerJ P Johnson
Mar 20, 1993·Lancet·K K TanabeH Saya
Aug 28, 1993·Lancet·S B FoxJ Fawcett
Jan 1, 1993·The Journal of Cell Biology·K H HeiderS T Pals
Feb 1, 1993·Cancer·D S Coffey
Dec 1, 1995·Journal of Neuro-oncology·L Y BourrguignonD Pasquale
Citations
Oct 1, 1996·Cancer·B V KallakuryJ S Ross
Jul 28, 1999·Cell Motility and the Cytoskeleton·L Y BourguignonY W Chen
Aug 26, 1998·Cancer Letters·S TakahashiT Shirai
Apr 25, 2003·Critical Reviews in Oncology/hematology·Tracey A MartinWen G Jiang
Apr 21, 1998·Critical Reviews in Oncology/hematology·S PrasadA Dritschilo
Dec 10, 2002·Hybridoma and Hybridomics·S M DavernS J Kennel
Jul 1, 2006·Bioinformatics·Dong WangBaofeng Yang
Apr 8, 1999·Journal of Clinical Pathology·V ParadisP Bedossa
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Melanie A Simpson, Vinata B Lokeshwar
Apr 21, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P LipponenV M Kosma
Jul 29, 2008·Connective Tissue Research·Shilenath GhatakSuniti Misra
Jul 8, 2009·Journal of Cellular Biochemistry·B DesaiM A Chellaiah
May 23, 2006·Biochimica Et Biophysica Acta·Th A ChristopoulosD H Vynios
Apr 14, 2016·International Journal of Molecular Sciences·Lilly Y W Bourguignon
Aug 1, 2014·Colloids and Surfaces. B, Biointerfaces·Soheyl Jafari MalekRassoul Dinarvand
Oct 21, 1998·Experimental Eye Research·M H Chaitin, A M Brun-Zinkernagel
Oct 15, 2013·Biomaterials·Anant ChopraPaul A Janmey
Dec 18, 2003·BJU International·H A H GadallaF G Elanany
May 29, 1998·Histopathology·K A IczkowskiD G Bostwick
Jul 23, 2004·Anti-cancer Drugs·Evan T Keller
Mar 31, 1998·Cell Motility and the Cytoskeleton·D Zhu, L Y Bourguignon
Sep 29, 1998·The Journal of Urology·H MiyakeS Kamidono
Jul 27, 2006·Genes, Chromosomes & Cancer·Ralph CarvalhoMarian A J Weterman
Jul 23, 2003·Cancer Gene Therapy·Lei ZhangGreg J Barritt
Sep 27, 1996·The Journal of Biological Chemistry·V B LokeshwarL Y Bourguignon
Mar 11, 1998·The Prostate·A M De MarzoG J Miller
Jul 9, 1999·The Prostate·A Y LiuG van den Engh
May 8, 1999·BJU International·C S FosterY Ke
May 16, 2007·The Journal of Biological Chemistry·Alamelu G BharadwajMelanie A Simpson
Feb 23, 2002·Clinical Orthopaedics and Related Research·Han-Soo KimSang Hoon Lee