Interaction of Chlamydia trachomatis with human genital epithelium in culture

Journal of General Microbiology
D R MoormanP B Wyrick

Abstract

Primary cultures of human endometrial and ectocervical epithelial cells were examined as a new model system to study genital infection by Chlamydia trachomatis. Initial studies demonstrated that these cells were indeed susceptible to chlamydial infection. Inocula, adjusted to produce inclusions in 50 to 80% of equivalent numbers of standard McCoy cells, resulted in infection rates of approximately 15 to 30% for the columnar cells of the endometrium and 5 to 10% for the squamous cells of the ectocervix. Exposure of cultures to DEAE-dextran and centrifugation-assisted inoculation, manipulations reported to enhance infection of HeLa and McCoy cells, did not alter the number of inclusion-positive genital cells. Addition of cycloheximide to the post-inoculation culture medium slightly increased numbers of inclusion-bearing cells while growth of genital cells in hormone-supplemented medium resulted in a variable effect on inclusion development and a significant reduction in the association of radiolabelled organisms with these cells. The basis for the different levels of infection in McCoy versus genital cell cultures was revealed by immunofluorescence analysis of chlamydial association with host cells immediately after inoculation. ...Continue Reading

Citations

Nov 1, 1995·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M E Ward
Jun 24, 2005·Infection and Immunity·Robert J SuchlandWalter E Stamm
Oct 2, 2002·Journal of Clinical Microbiology·Michael E BlockerMarcia M Hobbs
Oct 1, 1988·American Journal of Obstetrics and Gynecology·A S MaslowP B Wyrick
Jan 27, 2009·The Journal of Adolescent Health : Official Publication of the Society for Adolescent Medicine·Loris Y HwangAnna-Barbara Moscicki
Aug 25, 2007·Current Problems in Pediatric and Adolescent Health Care·Erica MonasterioMary-Ann Shafer
Feb 1, 1996·American Journal of Obstetrics and Gynecology·R P HeineP F Sparling
May 18, 2016·Epidemiology and Infection·F Y S KongJ S Hocking
Jan 1, 2020·Current Clinical Microbiology Reports·Amy Berry, Jennifer V Hall
Oct 27, 2017·Frontiers in Cellular and Infection Microbiology·Ana T NogueiraRey A Carabeo

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