Interaction of copper (II) complexes by bovine serum albumin: spectroscopic and calorimetric insights

Journal of Biomolecular Structure & Dynamics
Namrata SinghNand Kishore

Abstract

Serum albumins being the most abundant proteins in the blood and cerebrospinal fluid are significant carriers of essential transition metal ions in the human body. Studies of copper (II) complexes have gained attention because of their potential applications in synthetic, biological, and industrial processes. Study of binding interactions of such bioinorganic complexes with serum albumins improves our understanding of biomolecular recognition process essential for rational drug design. In the present investigation, we have applied quantitative approach to explore interactions of novel synthesized copper (II) complexes viz. [Cu(L1)(L2)ClO4] (complex I), [Cu(L2)(L3)]ClO4] (complex II) and [Cu(L4)2(H2O)2] (complex III) with bovine serum albumin (BSA) to evaluate their binding characteristics, site and mode of interaction. The fluorescence quenching of BSA initiated by complexation has been observed to be static in nature. The binding interactions are endothermic driven by entropic factors as confirmed by high sensitivity isothermal titration calorimetry. Changes in secondary and tertiary structure of protein have been studied by circular dichroism and significant reduction in α-helical content of BSA was observed upon binding. Sit...Continue Reading

References

Feb 6, 2004·Applied Microbiology and Biotechnology·J Spízek, T Rezanka
May 13, 2004·Journal of Pharmaceutical and Biomedical Analysis·Jiaqin LiuXingguo Chen
Nov 28, 2006·The Journal of Physical Chemistry. B·Tuhina BanerjeeNand Kishore
Dec 19, 2007·Antimicrobial Agents and Chemotherapy·Efthymia PetinakiRoland Leclercq
Aug 22, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yongnian NiSerge Kokot
Jun 3, 2009·Journal of Inorganic Biochemistry·Magdalena SokołowskaWojciech Bal
Aug 16, 2011·Drug Discovery Today·Yuko Kawasaki, Ernesto Freire
Jun 5, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Gopalaswamy VigneshRathinam Arthur James
May 15, 2013·Biochimica Et Biophysica Acta·Keishi YamasakiMasaki Otagiri
Aug 1, 1973·Molecular Biology Reports·G J Strous, H Bloemendal
Aug 5, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lijun YangLei Zhang
Jan 1, 2014·Clujul medical : revistă de medicină şi farmacie·Tamara TopalăRadu Oprean
Apr 26, 2016·Journal of Biomolecular Structure & Dynamics·Laxmi N JattinagoudarShivamurti A Chimatadar
May 22, 2016·Journal of Biomolecular Structure & Dynamics·Mohammad Rehan AjmalRizwan Hasan Khan

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Citations

Sep 16, 2017·Journal of Biomolecular Structure & Dynamics·Nuriye AkbayElmas Gökoğlu
Jul 28, 2018·Journal of Biomolecular Structure & Dynamics·Hamid TanzadehpanahMassoud Saidijam
Jul 28, 2018·Journal of Biomolecular Structure & Dynamics·Leila RoufegarinejadAli Jahanban-Esfahlan
Mar 5, 2019·Journal of Biomolecular Structure & Dynamics·Fatemeh Abyar, Leila Tabrizi
May 28, 2019·Journal of Biomolecular Structure & Dynamics·Zahra Aramesh-BoroujeniMeissam Noroozifar
May 31, 2019·Journal of Biomolecular Structure & Dynamics·Selvan NehruSelvakumar Veeralakshmi
Nov 7, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Damiano CirriAlessandro Pratesi
May 18, 2019·Journal of Biomolecular Structure & Dynamics·Srishti SharmaKallol K Ghosh

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Methods Mentioned

BETA
X-ray
isothermal titration
circular dichroism
fluorescence spectroscopy
fluorescence titration
Isothermal
Differential Scanning Calorimetery

Software Mentioned

Origin
IBH DAS

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