Interaction of copper with cysteine: stability of cuprous complexes and catalytic role of cupric ions in anaerobic thiol oxidation

Journal of Inorganic Biochemistry
Adelio RigoMarina Scarpa

Abstract

Copper complexes with cysteine have been investigated by optical spectroscopy, NMR and ESR. Cuprous ions strongly bind to the thiol group of Cys forming polymeric species with bridging thiolate sulfur according to a stoichiometry of about 1:1.2 and stability constant of the order of 10(10) M(-1). Cupric ions in the presence of cysteine, up to a ratio 0.45:1, are reduced to Cu(I) with stoichiometric production of cystine. The Cu(I) produced by this reaction is complexed by the excess of Cys. Trace amounts of Cu(II) exceeding the ratio 0.45:1 induce fast and complete oxidation of the Cys-Cu(I) complex to cystine with concomitant production of Cu(0) which precipitates. The experimental data are consistent with a mechanism by which Cu(II) oxidizes the complex Cys-Cu(I) to cystine producing aqueous Cu(I) which undergoes dismutation regenerating Cu(II). According to this mechanism the uncomplexed Cu(II) plays a catalytic oxidative role in the absence of molecular oxygen. The biological significance of these reactions is discussed.

References

Mar 1, 1996·European Journal of Biochemistry·A CorazzaP J Sadler
Nov 14, 1997·Journal of Inorganic Biochemistry·L C Tran-HoG T Hefter
May 19, 1998·Trends in Biochemical Sciences·C Askwith, J Kaplan
Dec 22, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·A CorazzaM Scarpa
Mar 17, 2000·Free Radical Biology & Medicine·D P JonesP Sternberg
Jul 27, 2001·Brain Research Bulletin·D StrausakG Multhaup
Jun 1, 2002·Current Opinion in Chemical Biology·Sergi Puig, Dennis J Thiele
Sep 6, 2002·Biochemical Pharmacology·Giuseppe FilomeniMaria Rosa Ciriolo
Sep 7, 2002·The Journal of Biological Chemistry·Christa J MaynardQiao-Xin Li
Aug 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·D Larry Sparks, Bernard G Schreurs
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ashley I BushRudolph E Tanzi
Dec 4, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H TapieroK D Tew

❮ Previous
Next ❯

Citations

Feb 3, 2016·Journal of the American Chemical Society·Sunghee KimStephen J Lippard
Aug 19, 2007·Nitric Oxide : Biology and Chemistry·Jean-François RoyAnn M English
Mar 25, 2015·Journal of Mass Spectrometry : JMS·DongWon ChoiLaurence A Angel
Oct 27, 2006·Biochimica Et Biophysica Acta·Ying YangJosé M Argüello
Apr 26, 2016·Biotechnology Progress·Jennifer PurdieDonald Olson
Feb 22, 2017·Journal of Agricultural and Food Chemistry·Matthias BaldusFrank-Jürgen Methner
Mar 8, 2017·Biotechnology Progress·Jennifer KuschelewskiAline Zimmer
Dec 4, 2014·Colloids and Surfaces. B, Biointerfaces·Michaela ŠimšíkováTomáš Šikola
Aug 17, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Duraiyarasu MaheshwaranRamasamy Mayilmurugan
May 31, 2018·Antimicrobial Agents and Chemotherapy·Richard J WallSusan Wyllie
Jan 12, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Selvarasu PriyangaRamasamy Mayilmurugan
Jan 24, 2008·Chemistry : a European Journal·Stefan StadlbauerBurkhard König
Jul 9, 2008·Journal of Microencapsulation·Nabil El KatebGilles Dumortier
May 10, 2015·EMBO Reports·Daniel MattleGabriele Meloni
Mar 7, 2014·Molecules and Cells·Bo-Young YoonNam-Chul Ha
Jul 11, 2019·Applied Microbiology and Biotechnology·Feixue LiuGeoffrey Michael Gadd
Apr 25, 2020·Molecular Microbiology·Andrea Giachino, Kevin J Waldron
Sep 3, 2020·Analytical and Bioanalytical Chemistry·Mohandoss SonaimuthuHui-Fen Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.