Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

Experimental Cell Research
Yansong LiXi Zhan

Abstract

Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.

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Citations

Feb 1, 2008·Journal of Clinical Lipidology·Jerzy-Roch Nofer
Dec 11, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Hiroaki YokomoriToshifumi Hibi
Jul 11, 2014·Biochimica Et Biophysica Acta·Brent A Wilkerson, Kelley M Argraves
Aug 29, 2006·Journal of Bone and Mineral Metabolism·Takuma MatsubaraToshiyuki Yoneda
Jan 20, 2005·American Journal of Physiology. Renal Physiology·Gürkan SengoelgeBarbara J Ballermann
May 30, 2019·Physiological Reviews·Nina WettschureckStefan Offermanns
Jul 7, 2017·Pulmonary Circulation·Patrick BelvitchSteven M Dudek
Sep 15, 2005·The Journal of Biological Chemistry·Jian-Jiang HaoXi Zhan
Dec 17, 2009·Experimental Cell Research·Dan YuXi Zhan

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