PMID: 11912229Mar 26, 2002Paper

Interaction of cytosolic and plastidic nitrogen metabolism in plants

Journal of Experimental Botany
Andreas Weber, Ulf-Ingo Flügge

Abstract

In angiosperms, the assimilation of ammonia resulting from nitrate reduction and from photorespiration depends on the operation of the plastidic GS/GOGAT cycle. The precursor for ammonia assimilation, 2-oxoglutarate, is synthesized in the mitochondria and in the cytosol. It is imported into the plastid by a 2-oxoglutarate/malate translocator (DiT1). In turn, the product of ammonia assimilation, glutamate, is exported from the plastids by a glutamate/malate translocator (DiT2). These transport processes link plastidic and cytosolic nitrogen metabolism and are essential for plant metabolism. DiT1 was purified to homogeneity from spinach chloroplast envelope membranes and identified as a protein with an apparent molecular mass of 45 kDa. Peptide sequences were obtained from the protein and the corresponding cDNA was cloned. The function of the DiT1 protein and its substrate specificity were confirmed by expression of the cDNA in yeast cells and functional reconstitution of the recombinant protein into liposomes. Recent advances in the molecular cloning of DiT2 and in the analysis of the in vivo function of DiT1 by antisense repression in transgenic tobacco plants will be discussed. In non-green tissues, the reducing equivalents re...Continue Reading

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Citations

Jan 1, 2002·The Arabidopsis Book·Uwe Ludewig, Wolf B Frommer
Jul 13, 2006·Planta·Rainer Hedrich, Irene Marten
Sep 7, 2005·Photosynthesis Research·Francisco R CantónFrancisco M Cánovas
May 6, 2008·Molecular & Cellular Proteomics : MCP·Wojciech MajeranKlaas J van Wijk
Mar 30, 2004·Journal of Experimental Botany·Andreas P M WeberLars M Voll
Apr 23, 2011·Journal of Experimental Botany·Ulf-Ingo FlüggeMarkus Gierth
Sep 15, 2011·Journal of Experimental Botany·Sobia IkramFabien Chardon
Dec 13, 2005·Plant Physiology·Lars M VollAndreas P M Weber
Sep 22, 2010·Plant Physiology·Andrea Bräutigam, Andreas P M Weber
May 3, 2005·Annual Review of Plant Biology·Andreas P M WeberUlf-Ingo Flügge
Oct 9, 2007·Genome Biology·Heather M TyraDebashish Bhattacharya
Mar 5, 2011·Progress in Lipid Research·Mie Shimojima
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Petra RennéAndreas P M Weber
Dec 24, 2010·The Plant Journal : for Cell and Molecular Biology·Hiromu KinoshitaMitsutaka Taniguchi
Mar 20, 2013·Plant Biology·M HodgesG Tcherkez
May 12, 2004·Current Opinion in Plant Biology·Andreas P M Weber
Sep 7, 2005·Trends in Plant Science·Marc Linka, Andreas P M Weber
Dec 30, 2009·Molecular Plant·Nicole Linka, Andreas P M Weber
Oct 19, 2006·Biochimica Et Biophysica Acta·Sigrun Reumann, Andreas P M Weber
May 27, 2003·Molecular & Cellular Proteomics : MCP·Myriam FerroNorbert Rolland
Oct 7, 2005·Molecular & Cellular Proteomics : MCP·Jean-Benoit PeltierKlaas J van Wijk
May 29, 2014·Molecular & Cellular Proteomics : MCP·Stéphanie ArrivaultWaltraud X Schulze
Jun 23, 2018·Plant Biology·J Selinski, R Scheibe
Mar 2, 2005·The Journal of Biological Chemistry·Michaela LerochJoachim Tjaden

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