Interaction of DNA with the movement proteins of geminiviruses revisited

Journal of Virology
Stefan HehnleHolger Jeske

Abstract

Geminiviruses manage the transport of their DNA within plants with the help of three proteins, the coat protein (CP), the nuclear shuttle protein (NSP), and the movement protein (MP). The DNA-binding capabilities of CP, NSP, and MP of Abutilon mosaic virus (AbMV; family Geminiviridae; genus Begomovirus) were scrutinized using gel mobility shift assays and electron microscopy. CP and NSP revealed a sequence-independent affinity for both double-stranded and single-stranded DNA, as has been previously reported for other begomoviruses. MP interacted selectively with dimeric supercoiled plasmid DNA in the electrophoretic assay. Further apparent size- and form-selective binding capacities of MP have been previously reported for another geminivirus (Bean dwarf mosaic virus), but in the case of AbMV, they have been identified as the result of electrophoretic interference rather than of complex formation. Without these complications, electron microscopy confirmed the assembly of double-stranded supercoiled DNA with NSP and MP into conspicuous structures and provided the first direct evidence for cooperative interaction of MP, NSP, and DNA. Based on these results and previous ones, a transport model of geminiviruses is discussed in which...Continue Reading

References

Sep 1, 1989·The Journal of General Virology·M I BoultonJ W Davies
Jun 10, 1988·Nucleic Acids Research·P EtessamiJ Stanley
Jan 1, 1981·CRC Critical Reviews in Biochemistry·C Brack
Oct 1, 1993·Virology·T FrischmuthJ Stanley
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·T KunikY Gafni
Feb 13, 2001·Virology·W ZhangR McKenna
Apr 18, 2002·FEBS Letters·Luke A SelthM Ali Rezaian
Feb 14, 2003·Journal of Virology·Werner Preiss, Holger Jeske
Jun 15, 2004·Journal of Virology·Bettina BöttcherHolger Jeske
Oct 24, 2008·Phytopathology·R G Guevara-GonzálezR F Rivera-Bustamante

❮ Previous
Next ❯

Citations

Aug 16, 2011·Virus Genes·Sukumaran SunithaKaruppannan Veluthambi
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Francisco García-HerasMontserrat Elías-Arnanz
Apr 15, 2005·Molecular Plant-microbe Interactions : MPMI·Jeanmarie Verchot-Lubicz
Oct 2, 2007·Plant Physiology·Yuval PeretzIlan Sela
Feb 5, 2010·Journal of Virology·Jesús Sánchez-NavarroLivia Stavolone
Aug 13, 2011·Journal of Virology·T PaprotkaH Jeske
Aug 5, 2005·Annual Review of Phytopathology·Maria R RojasRobert L Gilbertson
Jun 6, 2009·FEBS Letters·Muhammad Shah Nawaz-ul-Rehman, Claude M Fauquet
Feb 21, 2007·Plant Biotechnology Journal·Hervé VanderschurenPeng Zhang
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
Jul 14, 2010·Molecular Plant Pathology·Avner Levy, Tzvi Tzfira
Jul 3, 2007·Virus Research·Juan JovelHolger Jeske
Jan 10, 2018·Frontiers in Plant Science·Liping WangRosa Lozano-Duran

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.