PMID: 6162476Dec 23, 1980Paper

Interaction of Escherichia coli host factor protein with oligoriboadenylates

Biochemistry
P L de Haseth, O C Uhlenbeck

Abstract

The interaction of Escherichia coli host factor 1 with oligoadenylate [oligo(A)] was studied by fluorescence and filter retention techniques. The intrinsic fluorescence of the host factor is quenched by up to 60% by the addition of oligo(A). Fluorescence titrations at high protein concentrations (6 microM) give a saturation point of 14 A residues per host factor hexamer regardless of chain length or ionic strength. Nitrocellulose filter retention experiments at much lower concentrations (1 nM) indicate equimolar complexes form between (pA)l (12 less than l less than 27) and host factor hexamers. The smallest number of contiguous A residues which allows the formation of all favorable protein--RNA contacts is 16 at both low and high salt concentrations. At 0.1 M NaCl, the molar association constants are in the range of 10(10)--10(11) M-1 (15 less than l less than 27) and decrease only slightly with ionic strength, indicating a large nonionic component in the interaction. Cyclized (pA)l was shown to have a higher affinity for host factor than its linear counterparts when l is 18 or greater but a lower relative affinity when l is 15. This suggests that the binding site on the hexamer has a circular spatial orientation.

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Dec 23, 1980·Biochemistry·P L de Haseth, O C Uhlenbeck
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Citations

Dec 20, 1988·Journal of Molecular Biology·D BeckettO C Uhlenbeck
Nov 9, 2004·Nature Structural & Molecular Biology·Peter J MikuleckyAndrew L Feig
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Todd M LinkRichard G Brennan
Oct 25, 1995·Nucleic Acids Research·A NemethE Wahle
Dec 5, 2003·Nucleic Acids Research·Marc FolichonEliane Hajnsdorf
Mar 8, 2011·Nucleic Acids Research·Julia F HopkinsSarah A Woodson
Sep 12, 2012·Nucleic Acids Research·Nicola HorstmannRichard G Brennan
Jun 19, 2013·Nucleic Acids Research·Subrata PanjaSarah A Woodson
Dec 3, 2010·Genes & Development·Aurélie FenderE Gerhart H Wagner
Jan 6, 2011·RNA·Taylor B UpdegroveRoger M Wartell
Nov 30, 2013·Nucleic Acids Research·Kirsten E RobinsonRichard G Brennan
Mar 23, 2012·Critical Reviews in Microbiology·Patricio Sobrero, Claudio Valverde
Feb 14, 2012·Journal of Molecular Biology·Subrata Panja, Sarah A Woodson
Oct 5, 2010·Journal of Molecular Biology·Anders Steno OlsenPoul Valentin-Hansen
Dec 20, 2015·Future Microbiology·Joana R FelicianoJorge H Leitão
Mar 31, 2007·Current Opinion in Microbiology·Richard G Brennan, Todd M Link
Mar 11, 2004·Molecular Microbiology·Poul Valentin-HansenChristina Udesen
Nov 25, 2004·Journal of Molecular Biology·Richard A Lease, Sarah A Woodson
Apr 10, 2012·Journal of Molecular Biology·Helen A VincentAnastasia J Callaghan
Apr 11, 2018·Wiley Interdisciplinary Reviews. RNA·Andrew Santiago-Frangos, Sarah A Woodson
Jul 16, 2011·Nature Reviews. Microbiology·Jörg Vogel, Ben F Luisi
Sep 17, 2015·The EMBO Journal·Daniel J SchuGisela Storz
May 12, 2015·Angewandte Chemie·Subrata PanjaSarah A Woodson
Jun 15, 1983·Journal of Molecular Biology·S J RoseO C Uhlenbeck

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