Interaction of Escherichia coli K1 and K5 with Acanthamoeba castellanii trophozoites and cysts.

The Korean Journal of Parasitology
Abdul Matin, Suk-Yul Jung

Abstract

The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to understand the interactions of Escherichia coli K1 and E. coli K5 strains with Acanthamoeba castellanii trophozoites and cysts, association assay, invasion assay, survival assay, and the measurement of bacterial numbers from cysts were performed, and nonpathogenic E. coli K12 was also applied. The association ratio of E. coli K1 with A. castellanii was 4.3 cfu per amoeba for 1 hr but E. coli K5 with A. castellanii was 1 cfu per amoeba for 1 hr. By invasion and survival assays, E. coli K5 was recovered less than E. coli K1 but still alive inside A. castellanii. E. coli K1 and K5 survived and multiplied intracellularly in A. castellanii. The survival assay was performed under a favourable condition for 22 hr and 43 hr with the encystment of A. castellanii. Under the favourable condition for the transformation of trophozoites into cysts, E. coli K5 multiplied significantly. Moreover, the pathogenic potential of E. coli K1 from A. castellanii cysts exhibited no changes as compared with E. coli K1 from A. ca...Continue Reading

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Citations

Apr 15, 2014·Experimental Parasitology·David Lloyd
Jun 14, 2015·Applied and Environmental Microbiology·Ellen LambrechtKurt Houf
Jan 9, 2015·Clinical Microbiology Reviews·Jean-Christophe LagierDidier Raoult
Feb 6, 2020·Journal of Applied Microbiology·A A Gabriel, D C Panaligan
Jul 8, 2021·Applied and Environmental Microbiology·Félix BornierXavier Charpentier

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