Interaction of Ganoderma triterpenes with doxorubicin and proteomic characterization of the possible molecular targets of Ganoderma triterpenes

Cancer Science
Qing-Xi YueDe-An Guo

Abstract

Triterpenes are the main components with cytotoxicity in Ganoderma lucidum, which is used popularly as a complementary treatment for cancer therapy in traditional Chinese medicine. To investigate the possible interaction between chemotherapeutic agents and triterpenes extracted from G. lucidum, the cytotoxicity of doxorubicin (DOX) combined with Ganoderma triterpenes (GTS) or lucidenic acid N (LCN), a purified compound, was examined in HeLa cells. The combinations targeting DOX with GTS or LCN resulted in a synergistic interaction in HeLa cells. Moreover, to identify the molecular targets of GTS, two-dimensional gel electrophoresis-based comparative proteomics was carried out and proteins with altered expression levels after GTS treatment in HeLa cells were identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. The results of our proteomic study indicated that the GTS treatment caused regulated expression of 14 proteins, which play important roles in cell proliferation, the cell cycle, apoptosis, and oxidative stress. Flow cytometric analysis confirmed that GTS could induce weak G(0)-G(1) phase arrest and combined use of GTS with DOX could induce apoptosis in cells. Furthermore, GTS e...Continue Reading

References

Aug 1, 1998·Biochimica Et Biophysica Acta·H ShenK D Tew
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Feb 11, 2000·Annals of the New York Academy of Sciences·T K Yun
Apr 4, 2001·The Journal of Biological Chemistry·S F ShihJ Y Lin
Aug 25, 2001·Journal of Natural Products·T S WuS C Kuo
Oct 25, 2002·International Journal of Cancer. Journal International Du Cancer·Hongbo HuKyung-Sun Kang
Dec 4, 2003·Apoptosis : an International Journal on Programmed Cell Death·C C YuanL C Tsai
Jan 1, 1997·Apoptosis : an International Journal on Programmed Cell Death·C C YuanT S Huang
Nov 17, 2004·Mini Reviews in Medicinal Chemistry·Daniel Sliva
Mar 23, 2005·Biochemical and Biophysical Research Communications·Gwenaelle StanleyDaniel Sliva
Dec 15, 2005·Nutrition and Cancer·John W M Yuen, Mayur Danny I Gohel
Dec 27, 2005·Drug Discovery Today·Nicole Johnston
Jan 21, 2006·Leukemia Research·Claudia I MüllerH Phillip Koeffler
Jan 24, 2006·International Immunopharmacology·Xiao ChenShu-Feng Zhou
Feb 7, 2006·Leukemia Research·Daniel Sliva
May 16, 2006·Seminars in Cancer Biology·Heiko Hermeking, Anne Benzinger
May 24, 2006·The Journal of Biological Chemistry·Stephen S LinThomas M Roberts
Jan 29, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chao MaDe-An Guo

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Citations

Oct 25, 2012·Journal of Natural Products·José-Luis RíosRosa-María Giner
Jun 25, 2013·Expert Opinion on Investigational Drugs·Guo-Sheng WuYi-Tao Wang
Aug 5, 2009·International Immunopharmacology·Shailesh DudhgaonkarDaniel Sliva
May 9, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chao MaDe-An Guo
Oct 16, 2012·Journal of Biomedical Materials Research. Part a·Da-Wen DongXian-Rong Qi
Sep 23, 2011·Biochemical Society Transactions·Xuan Liu, De-An Guo
Aug 1, 2012·Biochemical Pharmacology·Wamtinga Richard SawadogoMarc Diederich
Jan 30, 2015·Integrative Cancer Therapies·Shujie Cheng, Daniel Sliva
Jul 10, 2010·Phytochemistry·Chun-Ru ChengDe-An Guo
Apr 16, 2013·Food & Function·Kotamballi N Chidambara MurthyBhimanagouda S Patil
Sep 1, 2015·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Nebojša V KladarBiljana N Božin
Apr 10, 2021·The Journal of Pharmacy and Pharmacology·Jing XuHang Song
Jan 23, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Q-X YueD-A Guo

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