Interaction of granulocytes and endothelial cells upon stimulation with tumor necrosis factor-alpha: an ultrastructural study

Immunobiology
G Zeck-KappU N Riede

Abstract

By the production of microbicidal agents, such as reactive oxygen species, activated PMN are capable of inducing tissue damage in the host. TNF-alpha was recently shown to be a potent activator of PMN oxidative metabolism. To further evaluate the interaction between activated PMN with physiological target cells, the effect of human PMN on cultured bovine aortic and human umbilical vein endothelial cells (EC) upon stimulation with human TNF-alpha was investigated by ultrastructural techniques: Scanning and transmission electron microscopy (SEM and TEM resp.) and ultrastructural detection of H2O2 production. When isolated PMN were added to EC in the presence of recombinant human TNF-alpha (10(3) U/ml) the EC-monolayer was disrupted within 4 h and EC changed their shape by exhibiting a spindle-like structure. PMN were seen in the intercellular spaces. Release of H2O2 was observed at the surface of the PMN plasma membrane, the luminal part of the small intracytoplasmic vacuoles in the PMN as well as in the contact zone between PMN and EC, but not within the EC. Scavengers of reactive oxygen species, such as superoxide dismutase and catalase or D-mannitol failed to block the effect of TNF-alpha-stimulated PMN on EC. In contrast, add...Continue Reading

References

Jan 1, 1990·The Journal of Infectious Diseases·J G CannonB Chernow
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R GambleM A Vadas
Nov 1, 1981·The Journal of Clinical Investigation·A NakagawaraZ A Cohn

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Citations

Sep 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E ZaczyńskaE Gejdel
May 11, 2002·Critical Care Medicine·Lance S Terada
Aug 1, 1991·The British Journal of Dermatology·A Kapp, G Zeck-Kapp
Jul 13, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sahithi J KuraviSamantha P Tull

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