PMID: 7519057Jan 1, 1994Paper

Interaction of Hoechst 33258 with a DNA triple helix

Biochimie
M DurandJ C Maurizot

Abstract

The interaction of Hoechst 33258 molecule, a minor groove binding drug, with T-A-T triple helix and A-T double helix was studied using circular dichroism spectroscopy and thermal denaturation. The triple helix consisted of an oligonucleotide (dA)12-x-(dT)12-x-(dT)12, where x is a hexa-ethylene glycol chain bridged between the 3' phosphate of one strand and the 5' phosphate of the following strand. This oligonucleotide is able to fold back on itself to form a very stable triplex. Circular dichroism spectroscopy demonstrates that Hoechst 33258 can bind to the triple helical structure. Spectral analysis shows that the bound drug exhibits a conformation and an environment slightly different in double-stranded and in triple-stranded structure. The affinity to the triple stranded structure is found smaller than to the double stranded one. Thermal denaturation experiments demonstrate that Hoechst 33258 destabilizes the triplex whereas it stabilizes the duplex.

References

Jan 1, 1976·Annual Review of Biophysics and Bioengineering·S A Latt
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y W Park, K J Breslauer
Jul 11, 1985·Nucleic Acids Research·K D Harshman, P B Dervan
Sep 20, 1987·Journal of Molecular Biology·P E PjuraR E Dickerson
May 20, 1988·Journal of Molecular Biology·V Murray, R F Martin
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J C HanveyR D Wells

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Citations

Oct 30, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mahima Kaushik, Shrikant Kukreti
Nov 10, 2009·DNA and Cell Biology·Garima Singhal, Moganty R Rajeswari
Feb 24, 2009·Oligonucleotides·Akash K JainVibha Tandon
May 11, 2018·Physical Chemistry Chemical Physics : PCCP·Belinda J BoehmDavid M Huang
Sep 7, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Imee M A Del MundoGuliang Wang
Dec 2, 1999·Pharmacology & Therapeutics·B S ReddyJ W Lown
Jun 1, 2010·Bioconjugate Chemistry·Akash K Jain, Santanu Bhattacharya
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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