Interaction of host-coded and virus-coded polypeptides in RNA phage replication

Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character
T Blumenthal

Abstract

The enzymes responsible for replication of the RNA of the single-stranded RNA bacteriophages contain, in addition to one phage-coded polypeptide, three host-coded polypeptides taken from the protein biosynthetic machinery: ribosomal protein S1 and the elongation factors Tu and Ts. While S1 performs a function in RNA replication derived from its protein synthetic function, mRNA binding, the reactions catalysed by the elongation factors in protein synthesis are apparently dispensible for RNA replication. In the replicase, these polypeptides, acting as the EF-Tu . Ts complex, play a fundamental structural role. Replacement of the endogenous EF-Tu with mutant EF-Tu, itself stable, causes the RNA replicase to become unstable. The possibility that EF-Tu . Ts is solely a structural protein in the RNA replicase is suggested by experiments showing that a variety of modifications of the elongation factors can be tolerated without loss of RNA synthetic capacity. In fact, EF-Tu . Ts from distantly related bacterial species can substitute for E. coli EF-Tu . Ts in RNA replicase. Evidence is presented that the high in vitro template specificity of Q beta replicase may be accomplished through modulation of the level of GTP required for initia...Continue Reading

Citations

May 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tadas PanavasPeter D Nagy
Apr 16, 2008·Infection and Immunity·Sowmya BalasubramanianJoel B Baseman
Mar 9, 2002·Journal of Virology·Stéphane BressanelliRaffaele De Francesco
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·T DasA K Banerjee
Jun 20, 2006·Journal of Bioscience and Bioengineering·Hiroshi KitaTetsuya Yomo
Apr 1, 2017·Frontiers in Microbiology·Ying-Ping HuangChing-Hsiu Tsai
Aug 1, 1982·Parasitology·A A Newton

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