PMID: 6983488Nov 1, 1982Paper

Interaction of human alpha 1-proteinase inhibitor with chymotrypsinogen A and crystallization of a proteolytically modified alpha 1-proteinase inhibitor

Hoppe-Seyler's Zeitschrift für physiologische Chemie
H LöbermannR Huber

Abstract

Human alpha 1-proteinase inhibitor (alpha 1-PI) can form very stable complexes with chymotrypsinogen A or chymotrypsin if limited proteolysis by a contaminant proteinase is prevented with diisopropyl fluorophosphate. The contaminant proteinase cleaves the alpha 1-PI component in the alpha 1-PI-chymotrypsinogen A complex close to its N-terminus, between threonine-11 and aspartate-12 and the chymotrypsinogen A part between tyrosine-146 and threonine-147. By this modification the complex becomes unstable and dissociates into modified alpha 1-PI and neo-chymotrypsinogen A. A tritium labelling experiment shows that the contaminant proteinase is present in a 0.5-1.0% (w/w) ratio in the inhibitor preparation. These experiments indicate that alpha 1-PI is not a temporary inhibitor for these enzymes, as assumed by other authors. Isolated modified alpha 1-PI can be crystallized as tetragonal bipyramides from 2.6M sodium potassium phosphate pH 8.0. The crystals are suitable for three dimensional X-ray structure analysis. In spite of the cleavage of the susceptible peptide bond by chymotrypsinogen A, the C-terminal 3.6 kDa cleavage peptide remains tightly bound to the inhibitor by means of non-covalent interactions. In accordance with the ...Continue Reading

References

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Citations

Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Christine WidmerUlrich Baumann
Aug 24, 2002·Thyroid : Official Journal of the American Thyroid Association·Urszula PiotrowskaJ Paul Banga
Apr 2, 1984·European Journal of Biochemistry·A LaineA Hayem
Jun 2, 2006·Genome Biology·Ruby H P LawJames C Whisstock
May 16, 1994·FEBS Letters·A J SchulzeR A Engh
Apr 16, 1985·Biochemical and Biophysical Research Communications·A Hercz
Jun 9, 2007·EMBO Reports·Qingwei ZhangJames C Whisstock
Apr 26, 2007·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Thomas W Stief
May 17, 2005·Journal of Thrombosis and Haemostasis : JTH·W Bode
Jul 1, 1985·Biological Chemistry Hoppe-Seyler·D Vercaigne-MarkoA Hayem

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