PMID: 6166324Apr 14, 1981Paper

Interaction of human alpha-amylases with inhibitors from wheat flour

Biochimica Et Biophysica Acta
C M O'Connor, K F McGeeney

Abstract

The interaction of four purified alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) inhibitors with human salivary and pancreatic alpha-amylases was investigated. The inhibitory activity of the four proteins towards salivary alpha-amylase was significantly increased by pre-incubation of the enzyme with inhibitor before adding substrate. This effect was not observed with the inhibition of pancreatic alpha-amylase by inhibitors 1 and 2. Inhibition of both amylases was affected to different degrees by incubating starch with inhibitor prior to the addition of enzyme. Maltose, at concentrations which only slightly affected amylase activity, prevented the inhibition of both enzymes by all four inhibitors. Gel filtration studies on salivary amylase-inhibitor mixtures showed the formation of EI complexes on a mol-to-mol ratio. A similar complex between pancreatic alpha-amylase and inhibitor 4 was observed, though complex formation between pancreatic alpha-amylase and the other inhibitors was not clearly demonstrated.

Citations

Jan 1, 1985·Critical Reviews in Clinical Laboratory Sciences·J J Zakowski, D E Bruns
Sep 1, 1993·Asia-Oceania Journal of Obstetrics and Gynaecology·K NakamuraT Mori
May 30, 2008·Journal of Medicinal Chemistry·Elena Lo PiparoChieh Jason Chou

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