Interaction of insulin with colloidal ZnS quantum dots functionalized by various surface capping agents

Materials Science & Engineering. C, Materials for Biological Applications
Ghader HosseinzadehAli A Moosavi-Movahedi

Abstract

Interaction of quantum dots (QDs) and proteins strongly influenced by the surface characteristics of the QDs at the protein-QD interface. For a precise control of these surface-related interactions, it is necessary to improve our understanding in this field. In this regard, in the present work, the interaction between the insulin and differently functionalized ZnS quantum dots (QDs) were studied. The ZnS QDs were functionalized with various functional groups of hydroxyl (OH), carboxyl (COOH), amine (NH2), and amino acid (COOH and NH2). The effect of surface hydrophobicity was also studied by changing the alkyl-chain lengths of mercaptocarboxylic acid capping agents. The interaction between insulin and the ZnS QDs were investigated by fluorescence quenching, synchronous fluorescence, circular dichroism (CD), and thermal aggregation techniques. The results reveal that among the studied QDs, mercaptosuccinic acid functionalized QDs has the strongest interaction (∆G°=-51.50kJ/mol at 310K) with insulin, mercaptoethanol functionalized QDs destabilize insulin by increasing the beta-sheet contents, and only cysteine functionalized QDs improves the insulin stability by increasing the alpha-helix contents of the protein, and. Our results...Continue Reading

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Jan 27, 2016·International Journal of Biological Macromolecules·Ghader HosseinzadehAli A Moosavi-Movahedi
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Citations

Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shiv K SharmaRoger M Leblanc
Jan 7, 2021·Nanoscale·Madison MonahanBrandi M Cossairt

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