Interaction of interleukin 7 (IL-7) with glycosaminoglycans and its biological relevance

Cytokine
D ClarkeM Gordon

Abstract

We have investigated the binding of interleukin 7 (IL-7) to sulfated glycosaminoglycans and evaluated its biological consequences. IL-7 binds to heparin and heparan sulfate, to a lesser extent to dermatan sulfate and does not bind to chondroitin sulfate. It was eluted from heparin by 0.3-0.6 M NaCl and from heparan sulfate by < 0.3 M NaCl. We also measured the affinity of IL-7 for heparin using an affinity co-electrophoresis method and found an affinity of 25 nM. In spite of these findings, IL-7 does not bind to the S17 cell line which supports lymphopoiesis. However, addition of heparin to cultures of an IL-7-dependent pre-B cell line (2E8) inhibited IL-7-stimulated proliferation and IL-7 complexed with heparin was more resistant than free IL-7 to protease treatment. Taken together, these results suggest that heparin may act as a carrier for IL-7, blocking its interaction with target cells and protecting it from degradation during transit.

Citations

Mar 24, 2004·Critical Reviews in Oncology/hematology·Yanusz Wegrowski, Francois-Xavier Maquart
Sep 12, 2002·Biochimica Et Biophysica Acta·Christelle CeboJean-Pierre Zanetta
Nov 30, 2002·Seminars in Immunology·Heather E Fleming, Christopher J Paige
Nov 26, 2009·Nature Reviews. Immunology·Kensuke Takada, Stephen C Jameson
Nov 28, 2007·Immunology and Cell Biology·John D MillerLucile E Wrenshall
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sven LétourneauOnur Boyman
Mar 23, 2000·The Journal of Experimental Medicine·R K SuniaraJ J Owen
Jun 6, 2012·Rejuvenation Research·Wayne A MitchellRichard Aspinall
Jan 17, 2009·International Immunology·Elliott FallerPaul Macpherson
Dec 23, 2006·Annals of the Rheumatic Diseases·Joel A G van RoonFloris P J G Lafeber
May 16, 2000·Critical Reviews in Clinical Laboratory Sciences·R E Banks
Mar 17, 2012·Seminars in Immunology·Angela M Crawley, Jonathan B Angel
Sep 21, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Frederique PonchelVincent Goëb
Jun 20, 2008·Experimental Gerontology·Richard Aspinall, Wayne Mitchell
Nov 27, 2007·Virus Research·Claudio PoiesiNino Manca
Nov 17, 2012·Chemistry : a European Journal·Guillaume DesprasJean-Maurice Mallet
May 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Sean GillCharles W Frevert
May 26, 2004·Archives of Biochemistry and Biophysics·Keiko FukushimaKatsuko Yamashita
Dec 24, 2014·Arthritis Research & Therapy·Sarah M ChurchmanFrederique Ponchel
Aug 31, 2006·Experimental Hematology·Joao T BarataAngelo A Cardoso
Dec 13, 2005·Seminars in Immunology·Marion EspeliClaudine Schiff
Apr 14, 2005·Seminars in Immunology·Kimberly D Klonowski, Leo Lefrançois
Sep 8, 2009·Analytica Chimica Acta·Yuexi Wang, Julie A Stenken
Nov 17, 2011·Biochimie·Fuming ZhangRobert J Linhardt
Dec 14, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Steven A DezellMichael R Verneris
Dec 13, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Paolo SportolettiMauro Di Ianni
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J J OwenE J Jenkinson
Jan 13, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Inkyung KangCharles W Frevert
Oct 31, 2018·Journal of Leukocyte Biology·Laura E Collins, Linda Troeberg
Jan 24, 2019·The Journal of Biological Chemistry·Khue G NguyenDavid A Zaharoff
May 16, 2002·Blood·Terry J Fry, Crystal L Mackall
Jul 3, 2002·The Journal of Biological Chemistry·Jean-Pierre ZanettaGérard Vergoten
Mar 23, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela M CrawleyJonathan B Angel
Apr 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas A BazdarScott F Sieg

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