Interaction of Leishmania PTS2 receptor peroxin 7 with the glycosomal protein import machinery

Molecular and Biochemical Parasitology
Ana Victoria C PilarArmando Jardim

Abstract

Leishmania proteins containing a peroxisomal targeting signal sequence 2 (PTS2) are selectively trafficked to the glycosome by associating with the peroxin 7 receptor protein (PEX7). The L. major PEX7 (LmPEX7) encodes a approximately 41 kDa protein that exhibits limited sequence identity with PEX7 homologues from other eukaryotic organisms. Functional characterization of recombinant and native LmPEX7 revealed that this receptor bound the PTS2 protein fructose-1,6-bisphosphate aldolase. Moreover, LmPEX7 also formed a tight association with the Leishmania PEX5, the cytosolic PTS1 receptor, and PEX14, a glycosomal peripheral membrane protein required for protein import into the glycosome. Mapping studies revealed that the Leishmania PEX7 binds to a domain on LdPEX5 encompassing residues 111-148 and to a site on LdPEX14 spanning residues 120-148. Finally, subcellular localization studies revealed that Leishmania PEX7 has a dual distribution within the cytosolic compartment and glycosomal lumen.

Citations

Jan 13, 2012·The Journal of Biological Chemistry·Jan M BoitzBuddy Ullman
Oct 25, 2012·PLoS Neglected Tropical Diseases·Ines Lakhal-NaouarRobert C Duncan
Jun 20, 2012·Molecular and Biochemical Parasitology·Sandeep KaushikAnjali Ganjiwale
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Mar 19, 2014·The Biochemical Journal·Ana Victoria C PilarArmando Jardim
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Nov 22, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Markus Kunze
Jan 16, 2018·Journal of Proteome Research·Armando JardimChristoph H Borchers

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