Interaction of ligand-receptor system between stromal-cell-derived factor-1 and CXC chemokine receptor 4 in human prostate cancer: a possible predictor of metastasis

Biochemical and Biophysical Research Communications
Hideki MochizukiKenji Kamiya

Abstract

Interaction of ligand-receptor systems between stromal-cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) is closely involved in the organ specificity of cancer metastasis. We hypothesized that SDF-1-CXCR4 ligand-receptor system plays an important role in prostate cancer metastasis. To test this hypothesis, expression level of SDF-1 and CXCR4 was analyzed in prostate cancer (PC) cell lines (LNCaP, PC3, and DU145) and normal prostate epithelial cell line (PrEC). We also performed migration assay and MTT assay to investigate the chemotactic effect and growth-promoting effect of SDF-1 on DU145 and PC3 cells, respectively. Furthermore, we performed immunohistochemical analysis of CXCR4 expression in tissues from 35 cases of human prostate cancer. CXCR4 expression was detected in all three prostate cancer cell lines, but not in PrECs. SDF-1 significantly enhanced the migration of PC3 and DU145 cells in a dose-dependent manner, and anti-CXCR4 antibody inhibited this chemotactic effect. However, SDF-1 itself did not significantly stimulate the cell growth rate of prostate cancer cell lines. Positive CXCR4 protein was found in 20 out of 35 clinical PC samples (57.1%). Three patients with lung metastasis showed definitel...Continue Reading

References

Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Dec 5, 1998·Journal of Immunological Methods·J M WangS Su
Jan 5, 2000·The Journal of Clinical Investigation·D Y JoM A Moore
Dec 6, 2000·The Journal of Clinical Investigation·T PonomaryovT Lapidot
Sep 26, 2001·The Journal of Biological Chemistry·M M RobledoJ Teixidó
Jun 13, 2002·The Journal of Investigative Dermatology·Aimee S Payne, Lynn A Cornelius

❮ Previous
Next ❯

Citations

May 25, 2005·Der Urologe. Ausg. A·F Vom DorpA Eisenhardt
Jan 22, 2009·Der Urologe. Ausg. A·M SchenckE Gulbins
Sep 13, 2005·Cancer Immunology, Immunotherapy : CII·Ana CoelhoRui Medeiros
Jun 16, 2005·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Mahboobeh RazmkhahAbbas Ghaderi
Oct 14, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Georgios KallifatidisIngrid Herr
May 11, 2010·Thyroid : Official Journal of the American Thyroid Association·Liborio TorregrossaFulvio Basolo
Aug 2, 2013·Molecular Cancer·M Katie Conley-LaCombSreenivasa R Chinni
Apr 6, 2013·Critical Reviews in Eukaryotic Gene Expression·Nicole SalazarBal L Lokeshwar
Sep 10, 2008·PPAR Research·Cynthia Lee Richard, Jonathan Blay
Jun 8, 2006·Neoplasia : an International Journal for Oncology Research·Tobias EnglRoman A Blaheta
Feb 4, 2010·Biochimica Et Biophysica Acta·Ling DengQianqian Lei
Dec 17, 2008·Seminars in Cancer Biology·Hyunsuk ShimNobutaka Fujii
Jan 11, 2013·Journal of Cellular and Molecular Medicine·Barbara MognettiClaudia Penna
Feb 11, 2011·Cell Biology International·Qinwen WangZhengtang Chen
Oct 22, 2005·The Breast : Official Journal of the European Society of Mastology·Y-C SuC-Y Chai
Oct 3, 2014·International Journal of Cancer. Journal International Du Cancer·Hye Won LeeKyeung Min Joo
Mar 17, 2015·Journal of Cellular Biochemistry·Yongming Xi, Yan Chen
Aug 12, 2009·Current Opinion in Pharmacology·Bharat B Aggarwal, Prashasnika Gehlot
Nov 5, 2016·Molecular Cancer·M Katie Conley-LaCombSreenivasa R Chinni
Jan 22, 2015·The World Journal of Men's Health·Joo Yong LeeKang Su Cho
Sep 7, 2018·Journal of Cellular Physiology·Shirin AzizidoostNajmaldin Saki
Jun 15, 2018·World Journal of Clinical Oncology·Marina TusupSteve Pascolo
Dec 29, 2018·International Journal of Molecular Sciences·Elisabetta MarcuzziBianca Calì
Jul 17, 2007·The Journal of Biological Chemistry·Lesa A BegleyJill A Macoska
Feb 18, 2015·Frontiers in Immunology·Maria VelaLeonor Kremer
Jun 27, 2020·International Journal of Molecular Sciences·Timothy O Adekoya, Ricardo M Richardson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.