Abstract
ROR is one of large intergenic non-coding RNAs (lincRNAs) and acts as a strong negative regulator of p53 and a sponge for miR-145 to regulate diverse cancer progression. However, the interaction of ROR, p53 and miR-145 in lung cancer and its correlation with lung cancer stem cell (LCSC) signatures were not fully understood. Here, qRT-PCR analysis revealed that p53 and ROR were upregulated while miR-145 was downregulated in non-small cell lung cancer (NSCLC) tissues and LCSCs compared to their paired adjacent normal tissues and lung cancer cells (LCCs). Besides, high p53 level, low miR-145 level, and high ROR level were associated with poor prognosis in NSCLC. Then, we found that silencing ROR failed to change p53 mRNA level but promoted p53 protein level, suggesting a posttranscriptional regulation mechanism involved in si-ROR-mediated promotion of p53. While silencing p53 moderately downregulated ROR. Additionally, silencing ROR or p53 upregulated miR-145. However, silencing both ROR and p53 failed to make obvious change to miR-145 mRNA level and p53 protein level. And interestingly, miR-145 negatively regulated Oct4 and Sox2. We also found that silencing ROR and (or) p53 suppressed in vitro cell proliferation, migration and i...Continue Reading
Citations
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